Inhibitory effects of a novel aromatase inhibitor, YM511, on growth of endometrial explants and insulin-like growth factor-I gene expression in rats with experimental endometriosis
| Autor: | Taiki Nanya, Yoko Susaki, Masafumi Kudoh, Hisataka Shikama, Yukitaka Ideyama, Masamichi Mori |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Antineoplastic Agents Hormonal medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Ovariectomy Clinical Biochemistry Uterus Endometriosis Gene Expression Biology Endometrium Biochemistry Rats Sprague-Dawley Insulin-like growth factor Endocrinology Internal medicine medicine Animals RNA Messenger Enzyme Inhibitors Insulin-Like Growth Factor I Molecular Biology Aromatase inhibitor Aromatase Inhibitors Growth factor Cell Biology Triazoles medicine.disease Rats medicine.anatomical_structure Estrogen Molecular Medicine Female Leuprolide Explant culture |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 63(1-3) |
| ISSN: | 0960-0760 |
| Popis: | Estrogen deprivation therapy effectively prevents progress of endometriosis but the precise mechanism by which estrogen stimulates growth of endometriotic implants is still unknown. We examined effects of hypoestrogenic state induced by ovariectomy, gonadotropin-releasing hormone agonist (leuprolide) or aromatase inhibitor (YM511), on growth of experimental endometrial explant, a section of endometrium transplanted under the renal capsule, in rats. Ovariectomy gradually reduced the volume of endometrial explants for 21 days. YM511 (0.1 mg/kg) and leuprolide (1 mg/rat) completely reduced volume of endometrial explants but they differed widely in the onset of inhibitory action. YM511 prevented growth of explants on day 4 but leuprolide had no inhibitory effect until day 15. YM511 dose-dependently reduced volume of endometrial explants and its minimum effective dose was 0.04 mg/kg. Insulin-like growth factor-I (IGF-I) mRNA expression in endometrial explant and uterus was examined on day 4. YM511 decreased IGF-I expression in endometrial explant and uterus by 58% and 48%, respectively. Reductions of the extent of IGF-I expression by YM511 and ovariectomy were comparable. A significant correlation between the volume and IGF-I mRNA expression in endometrial explant suggests that local expression of this gene may play an important role in stimulating growth of endometrial explants. |
| Databáze: | OpenAIRE |
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