Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo

Autor: Fabian Wehnekamp, Thomas Misgeld, Gabriela Plucińska, Rachel Thong, Don C. Lamb
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
metabolism [Vertebrates]
Structural Biology and Molecular Biophysics
metabolism [Axons]
Tracking (particle physics)
Physics of Living Systems
0302 clinical medicine
Nanotechnology
methods [Microscopy
Confocal]
metabolism [Zebrafish]
Biology (General)
Zebrafish
Physics
Millisecond
Microscopy
Confocal
biology
General Neuroscience
General Medicine
16. Peace & justice
ddc
Mitochondria
Tools and Resources
Cell Tracking
Larva
Vertebrates
Medicine
mitochondria trafficking
fluorescence
methods [Imaging
Three-Dimensional]
methods [Cell Tracking]
Sensory Receptor Cells
QH301-705.5
Science
single particle tracking
Context (language use)
Sensory system
metabolism [Larva]
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Imaging
Three-Dimensional
Organelle
Animals
metabolism [Sensory Receptor Cells]
General Immunology and Microbiology
Molecular biophysics
Biological Transport
biology.organism_classification
metabolism [Mitochondria]
methods [Nanotechnology]
Axons
030104 developmental biology
Temporal resolution
Biophysics
ddc:600
030217 neurology & neurosurgery
Zdroj: eLife, Vol 8 (2019)
eLife 8, e46059 (2019). doi:10.7554/eLife.46059
eLife
DOI: 10.7554/eLife.46059
Popis: We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unprecedented spatiotemporal resolution over distances of >100 µm. Using these nanoscopic trajectory data, we discriminated five motional states: a fast and a slow directional motion state in both the anterograde and retrograde directions and a stationary state. The transition pattern revealed that, after a pause, mitochondria predominantly persist in the original direction of travel, while transient changes of direction often exhibited longer pauses. Moreover, mitochondria in the vicinity of a second, stationary mitochondria displayed an increased probability to pause. The capability of following and optically manipulating a single organelle with high spatiotemporal resolution in a living organism offers a new approach to elucidating their function in its complete physiological context.
Databáze: OpenAIRE
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