The effect of exposure to irrigant solutions on apical dentin biofilms in vitro
| Autor: | Frank J. Vertucci, Leandro R. Britto, Clay Walker, M.S. Clegg, Myriam Bélanger |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Endodontic therapy
Materials science Sodium Hypochlorite Dentistry Polysorbates Citric Acid Microbiology chemistry.chemical_compound Tooth Apex medicine Dentin Dental Pulp Necrosis Humans General Dentistry Periapical periodontitis Microbial Viability Root Canal Irrigants business.industry Chlorhexidine Biofilm Sem analysis medicine.disease In vitro medicine.anatomical_structure chemistry Sodium hypochlorite Biofilms Doxycycline Microscopy Electron Scanning business Periapical Periodontitis medicine.drug |
| Zdroj: | Journal of endodontics. 32(5) |
| ISSN: | 0099-2399 |
| Popis: | This study assessed the effectiveness of different concentrations of sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) (Vista Dental Products, Racine, WI), and BioPure MTAD (Dentsply Endodontics-Tulsa Dental, Tulsa, OK). Intracanal contents were collected from 10 patients diagnosed with chronic apical periodontitis. The samples were cultured on hemisections of root apices to generate a polymicrobial biofilm. Each biofilm was separately immersed in 6% NaOCl, 3% NaOCl, 1% NaOCl, 2% CHX, 1% NaOCl followed by BioPure MTAD, and sterile phosphate buffered solution (PBS). SEM analysis showed 6% NaOCl and 3% NaOCl were capable of disrupting and removing the biofilm; 1% NaOCl and 1% NaOCl followed by MTAD were capable of disrupting the biofilm, but not eliminating bacteria; 2% CHX was not capable of disrupting the biofilm. Viable bacteria could not be cultured from specimens exposed to 6% NaOCl, 2 % CHX, or 1% NaOCl followed by BioPure MTAD. These results indicate that 6% NaOCl was the only irrigant capable of both rendering bacteria nonviable and physically removing the biofilm. |
| Databáze: | OpenAIRE |
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