Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats
| Autor: | DongSheng Ouyang, Ye Zheng, HongHao Zhou, Xiaofang Jia, Yanling Feng, Lin Yin, QianQian Zhao, Lijun Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Liver Cirrhosis
Male Proteomics Proteome Blotting Western Molecular Sequence Data Protein Disulfide-Isomerases Gene Expression PDIA3 Biology General Biochemistry Genetics and Molecular Biology Masson's trichrome stain Pathogenesis Rats Sprague-Dawley Environmental Science(all) Gene expression Animals Humans Electrophoresis Gel Two-Dimensional Amino Acid Sequence General Environmental Science Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Reverse Transcriptase Polymerase Chain Reaction Flow Cytometry Molecular biology Immunohistochemistry Rats Blot Disease Models Animal Liver General Agricultural and Biological Sciences Percoll |
| Zdroj: | Science China. Life sciences. 57(3) |
| ISSN: | 1869-1889 |
| Popis: | Elucidation of the mechanisms of liver fibrogenesis is important to treat liver fibrosis. In this study, we established rat models of liver fibrosis with stages from 0–1, 2, and 3–4 to 4 at 2, 4, 6, and 8 weeks, respectively, by injection of pig serum. Liver fibrogenesis was detected by Masson’s trichrome staining. Rat non-parenchymal cells (NPCs) were enriched 4-fold by Percoll density gradient centrifugation. Protein extracts from NPCs were prepared at 4 and 8 weeks, separated by two-dimensional electrophoresis, and then stained with Coomassie Blue G-250. At 4 weeks, we identified 18 non-redundant differentially expressed proteins of which protein disulfide-isomerase associated protein 3 (PDIA3) and NDUV showed consistent expression at protein and mRNA levels from 4 to 8 weeks. PDIA3 was found to be down-regulated by Western blotting in the rat model and immunohistochemically in human liver. Our results revealed important aspects of the pathogenesis/progression of liver fibrosis and demonstrated important changes in protein expression levels of NPCs at various stages of liver fibrosis. |
| Databáze: | OpenAIRE |
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