Reflectance Spectroscopy for Non-Destructive Measurement and Genetic Analysis of Amounts and Types of Epicuticular Waxes on Onion Leaves
| Autor: | Michael J. Havey, Eduardo D. Munaiz, Philip A. Townsend |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
spectroscopy 010504 meteorology & atmospheric sciences QTL leaf biochemistry Pharmaceutical Science Fatty alcohol Quantitative trait locus 01 natural sciences Gas Chromatography-Mass Spectrometry Article epicuticular wax Analytical Chemistry Epicuticular wax lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry PLSR Drug Discovery Onions genetics Cultivar Physical and Theoretical Chemistry Spectroscopy onion 0105 earth and related environmental sciences Wax biology Chemistry Spectrum Analysis Organic Chemistry fungi food and beverages Chromosome Mapping hentriacontanone-16 biology.organism_classification Plant Leaves Horticulture Phenotype Chemistry (miscellaneous) visual_art Waxes visual_art.visual_art_medium Molecular Medicine Allium glossy Gas chromatography–mass spectrometry fatty alcohol 010606 plant biology & botany |
| Zdroj: | Molecules Volume 25 Issue 15 Molecules, Vol 25, Iss 3454, p 3454 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25153454 |
| Popis: | Epicuticular waxes on the surface of plant leaves are important for the tolerance to abiotic stresses and plant&ndash parasite interactions. In the onion (Allium cepa L.), the variation for the amounts and types of epicuticular waxes is significantly associated with less feeding damage by the insect Thrips tabaci (thrips). Epicuticular wax profiles are measured using used gas chromatography mass spectrometry (GCMS), which is a labor intensive and relatively expensive approach. Biochemical spectroscopy is a non-destructive tool for measurement and analysis of physiological and chemical features of plants. This study used GCMS and full-range biochemical spectroscopy to characterize epicuticular waxes on seven onion accessions with visually glossy (low wax), semi-glossy (intermediate wax), or waxy (copious wax) foliage, as well as a segregating family from the cross of glossy and waxy onions. In agreement with previous studies, GCMS revealed that the three main waxes on the leaves of a wild type waxy onion were the ketone hentriacontanone-16 (H16) and fatty alcohols octacosanol-1 (Oct) and triacontanol-1 (Tri). The glossy cultivar &ldquo Odourless Greenleaf&rdquo had a unique phenotype with essentially no H16 and Tri and higher amounts of Oct and the fatty alcohol hexacosanol-1 (Hex). Hyperspectral reflectance profiles were measured on leaves of the onion accessions and segregating family, and partial least-squares regression (PLSR) was utilized to generate a spectral coefficient for every wavelength and prediction models for the amounts of the three major wax components. PLSR predictions were robust with independent validation coefficients of determination at 0.72, 0.70, and 0.42 for H16, Oct, and Tri, respectively. The predicted amounts of H16, Oct, and Tri are the result of an additive effect of multiple spectral features of different intensities. The variation of reflectance for H16, Oct, and Tri revealed unique spectral features at 2259 nm, 645 nm, and 730 nm, respectively. Reflectance spectroscopy successfully revealed a major quantitative trait locus (QTL) for amounts of H16, Oct, and Tri in the segregating family, agreeing with previous genetic studies. This study demonstrates that hyperspectral signatures can be used for non-destructive measurement of major waxes on onion leaves as a basis for rapid plant assessment in support of developing thrips-resistant onions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |