Proteomics characterization of gastrokine 1-induced growth inhibition of gastric cancer cells
| Autor: | Guang-Rong Yan, Qing-Yu He, Song-Hui Xu, Xin-Feng Yin, Zi-Lu Tan |
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| Rok vydání: | 2011 |
| Předmět: |
Proteomics
Cell cycle checkpoint MAP Kinase Signaling System Peptide Hormones Blotting Western Down-Regulation Apoptosis Biology Transfection Biochemistry chemistry.chemical_compound Downregulation and upregulation Stomach Neoplasms Cell Line Tumor Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Protein Interaction Maps Phosphorylation RNA Small Interfering Databases Protein Protein kinase A Molecular Biology Cell Proliferation Kinase Cell growth Tumor Suppressor Proteins Cancer Cell Cycle Checkpoints Flow Cytometry medicine.disease Cell biology DNA-Binding Proteins Enzyme Activation Gene Expression Regulation Neoplastic chemistry Phosphopyruvate Hydratase Cancer cell Mitogen-Activated Protein Kinases Growth inhibition |
| Zdroj: | PROTEOMICS. 11:3657-3664 |
| ISSN: | 1615-9853 |
| DOI: | 10.1002/pmic.201100215 |
| Popis: | We previously used proteomics technology to globally identify gastric cancer-associated proteins and found that gastrokine 1 (GKN1) was dramatically underexpressed in gastric cancer tissues. Here, we further showed that GKN1 could inhibit cell growth and induce cell cycle arrest in gastric cancer cells. The activity of protein kinase PKCδ/θ was inhibited by GKN1, whereas the activity of ERK1/2 and JNK1/2 was increased by GKN1, suggesting that GKN1 induced growth inhibition of gastric cancer cells by synergistically regulating the activity of these protein kinases. Seventy-four proteins were found to be regulated by GKN1 by proteomics analysis, including α-enolase (ENO1) and Cathepsin D. Interestingly, ENO1 is an important hub in the protein-protein interaction network of the 74 differential proteins. Silencing of ENO1 resulted in growth inhibition and cell cycle arrest of gastric cancer cells, similar to the effect of GKN1 overexpression in cells, whereas ENO1 overexpression blocked GKN1-induced growth inhibition and cell cycle arrest. These observations suggested that ENO1 downregulation played an important role in GKN1-induced growth inhibition of gastric cancer cells. |
| Databáze: | OpenAIRE |
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