Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development
| Autor: | Hong Zhang, Mary C. Mullins, Sumei Lu, Lee Kapp, Elliott W. Abrams, Florence L. Marlow |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Blastomeres Embryo Nonmammalian Insecta Saccharomyces cerevisiae Proteins Nuclear Envelope Zygote Molecular Sequence Data Saccharomyces cerevisiae Spindle Apparatus Biology Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Animals Humans Nuclear fusion Inner membrane Amino Acid Sequence Nuclear protein Mitosis Zebrafish 030304 developmental biology Mammals Genetics 0303 health sciences Pronuclear fusion Biochemistry Genetics and Molecular Biology(all) Cell Cycle Membrane Proteins Nuclear Proteins Lipid bilayer fusion Zebrafish Proteins Embryo Mammalian Chromatin Cell biology Female Sequence Alignment 030217 neurology & neurosurgery Fusion mechanism |
| Zdroj: | Cell. 150:521-532 |
| ISSN: | 0092-8674 |
| Popis: | SummaryTo accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions. Karyomeres are one such modification, mitotic intermediates wherein individual chromatin masses are surrounded by nuclear envelope; the karyomeres then fuse to form a single mononucleus. We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion, resulting in formation of multiple micronuclei. As karyomeres form, Brambleberry protein localizes to the nuclear envelope, with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites. brambleberry corresponds to an unannotated gene with similarity to Kar5p, a protein that participates in nuclear fusion in yeast. We also demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish. Our studies provide insight into the machinery required for karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres. |
| Databáze: | OpenAIRE |
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