Efficacy assessment of self-assembled PLGA-PEG-PLGA nanoparticles: Correlation of nano-bio interface interactions, biodistribution, internalization and gene expression studies

Autor: Nikola Geskovski, Boguslaw Szczupak, Rozafa Koliqi, Jordi Llop, Aleksandar Dimovski, Sonja Ugarkovic, Katerina Goracinova, Delyan R. Hristov, Gjorgji Petruševski, Eneko San Sebastián, Nadica Matevska-Geskovska, Vanessa Gómez Vallejo, Marco P. Monopoli, Simona Dimchevska
Rok vydání: 2017
Předmět:
Biodistribution
Biocompatibility
Surface Properties
media_common.quotation_subject
Fibroblast Growth Factor 3
Muscle Proteins
Pharmaceutical Science
Nanoparticle
Cell Cycle Proteins
Nerve Tissue Proteins
Nanotechnology
02 engineering and technology
Irinotecan
010402 general chemistry
01 natural sciences
Polyethylene Glycols
Histones
Cell Line
Tumor
Zeta potential
Copolymer
Animals
Humans
Tissue Distribution
Particle Size
Rats
Wistar
Internalization
Polyglactin 910
Ubiquitins
media_common
Chemistry
technology
industry
and agriculture
Serum Albumin
Bovine
021001 nanoscience & nanotechnology
Antineoplastic Agents
Phytogenic
0104 chemical sciences
Gene Expression Regulation
Neoplastic
Molecular Weight
Biophysics
Nanoparticles
Camptothecin
Adsorption
Nanocarriers
0210 nano-technology
Protein adsorption
Zdroj: International Journal of Pharmaceutics. 533:389-401
ISSN: 0378-5173
Popis: The aim of our study was to develop and compare the biological performance of two types of biodegradable SN-38 loaded nanoparticles (NPs) with various surface properties, composed of low and high Mw triblock PLGA-PEG-PLGA copolymers, applying rational quality and safety by design approach. Therefore, along with the optimization of crucial physico-chemical properties and in order to evaluate the therapeutical potential and biocompatibility of prepared polymeric nanoparticles, analysis of nano-bio interactions, cell internalization, gene expression and biodistribution studies were performed. The optimized formulations, one of low Mw and one composed of high Mw PLGA-PEG-PLGA copolymer, exhibited different characteristics in terms of surface properties, particle size, zeta potential, drug loading, protein adsorption and biodistribution, which may be attributed to the variations in nano–bio interface interactions due to different NP building blocks length and Mw. On the contrary to protein adsorption and biodistribution studies, both types of NPs exhibited similar results during cell internalization and gene expression studies performed in cell culture medium containing serum proteins. This pool of useful data for internalization and efficacy as well as the notable advance in the circulation time of low Mw NPs may be further employed for shaping the potential of the designed nanocarriers.
Databáze: OpenAIRE
Externí odkaz: