Self-assembly of pericentriolar material in interphase cells lacking centrioles
| Autor: | Chen, Fangrui, Wu, Jingchao, Iwanski, Malina K, Jurriens, Daphne, Sandron, Arianna, Pasolli, Milena, Puma, Gianmarco, Kromhout, Jannes Z, Yang, Chao, Nijenhuis, Wilco, Kapitein, Lukas C, Berger, Florian, Akhmanova, Anna, Sub Cell Biology, Celbiologie |
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| Přispěvatelé: | Sub Cell Biology, Celbiologie |
| Rok vydání: | 2022 |
| Předmět: |
PLK4
animal structures Centriole NEDD1 Neuroscience(all) Microtubules Biochemistry General Biochemistry Genetics and Molecular Biology Microtubule Immunology and Microbiology(all) Animals CAMSAP skin and connective tissue diseases Interphase Centrioles Pericentriolar material dynein pericentrin General Immunology and Microbiology urogenital system Chemistry Biochemistry Genetics and Molecular Biology(all) General Neuroscience Dyneins pericentriolar material Microtubule organizing center General Medicine Cell biology Centrioles/metabolism Centrosome/metabolism microtubule-organizing center centrosome Microtubules/metabolism Centrosome embryonic structures Dyneins/metabolism Human microtubule Genetics and Molecular Biology(all) |
| Zdroj: | eLife, 11, 1. eLife Sciences Publications |
| ISSN: | 2050-084X |
| Popis: | The major microtubule-organizing center (MTOC) in animal cells, the centrosome, comprises a pair of centrioles surrounded by pericentriolar material (PCM), which nucleates and anchors microtubules. Centrosome assembly depends on PCM binding to centrioles, PCM self-association and dyneinmediated PCM transport, but the self-assembly properties of PCM in interphase cells are poorly understood. Here, we used experiments and modeling to study centriole-independent features of interphase PCM assembly. We showed that when centrioles are lost due to PLK4 depletion or inhibition, dynein-based PCM transport and PCM self-clustering are sufficient to form a single compact MTOC, which generates a dense radial microtubule array. Interphase PCM self-assembly depends on γ-tubulin, pericentrin, CDK5RAP2 and ninein, but not NEDD1, CEP152 or CEP192. Formation of a compact acentriolar MTOC is inhibited by AKAP450-dependent PCM recruitment to the Golgi or by randomly organized CAMSAP2-stabilized microtubules, which keep PCM mobile and prevent its coalescence. Linking of CAMSAP2 to a minus-end-directed motor leads to the formation of an MTOC, but MTOC compaction requires cooperation with pericentrin-containing self-clustering PCM. Our data reveal that interphase PCM contains a set of components that can self-assemble into a compact structure and organize microtubules, but PCM self-organization is sensitive to motor-and microtubule-based rearrangement. |
| Databáze: | OpenAIRE |
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