Identification and mapping of the QTL for aluminum tolerance introgressed from the new source, Oryza Rufipogon Griff., into indica rice (Oryza sativa L.)
| Autor: | Henry T. Nguyen, Buu C. Bui, Tao V. Nguyen, Darshan S. Brar, Luong N. Pham, Bay D. Nguyen |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
Positional cloning Genotype Genetic Linkage Population Quantitative Trait Loci Quantitative trait locus Biology Genetic analysis Plant Roots chemistry.chemical_compound Species Specificity Molecular marker Botany Genetics education Alleles Crosses Genetic Plant Diseases education.field_of_study Oryza sativa Polymorphism Genetic Models Genetic food and beverages Chromosome Mapping Epistasis Genetic Oryza General Medicine biology.organism_classification Oryza rufipogon Phenotype chemistry Epistasis Edible Grain Agronomy and Crop Science Biotechnology Aluminum |
| Zdroj: | TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 106(4) |
| ISSN: | 0040-5752 |
| Popis: | This study was conducted to identify and map the quantitative trait locus (QTL) controlling Al tolerance in rice using molecular markers. A population of 171 F(6) recombinant inbred lines (RILs) derived from the cross of Oryza sativa (IR64), the Al susceptible parent, and Oryza rufipogon, the Al tolerant parent, was evaluated for Al tolerance using a nutrient solution with and without 40 ppm of active Al(+3). A genetic map, consisting of 151 molecular markers covering 1,755 cM with an average distance of 11.6 cM between loci, was constructed. Nine QTLs were dentified including one for root length under non-stress conditions (CRL), three for root length under Al stress (SRL) and five for relative root length (RRL). O. rufipogon contributed favorable alleles for each of the five QTLs for RRL, which is a primary parameter for Al tolerance, and individually they explained 9.0-24.9% of the phenotypic variation. Epistatic analysis revealed that CRL was conditioned by an epistatic effect, whereas SRL and RRL were controlled by additive effects. Comparative genetic analysis showed that QTLs for RRL, which mapped on chromosomes 1 and 9, appear to be consistent among different rice populations. Interestingly, a major QTL for RRL, which explained 24.9% of the phenotypic variation, was found on chromosome 3 of rice, which is conserved across cereal species. These results indicate the possibilities to use marker-assisted selection and pyramiding QTLs for enhancing Al tolerance in rice. Positional cloning of such QTLs introgressed from O. rufipogon will provide a better understanding of the Al tolerance mechanism in rice and the evolutionary genetics of plant adaptation to acid-soil conditions across cereal species. |
| Databáze: | OpenAIRE |
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