Mutagenicity of cyclopenta-fused polynuclear aromatic hydrocarbons and a non- polar fraction from a fuel combustion sample in a Salmonella forward mutation assay without exogenous metabolic activation
| Autor: | Patrick P. J. Mulder, Jan Cornelisse, Arthur L. Lafleur, William F. Busby, Elaine F. Plummer, Henrietta Smith, Johan Lugtenburg, Ben B. Boere |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Salmonella typhimurium
endocrine system Salmonella Ethylene Health Toxicology and Mutagenesis Fraction (chemistry) Mutagen Mitochondria Liver Instituut voor Agrotechnologisch Onderzoek medicine.disease_cause Methylation Toxicology chemistry.chemical_compound Structure-Activity Relationship Genetics medicine Potency Animals Life Science Polycyclic Aromatic Hydrocarbons Anthracenes Mutation Chromatography Dose-Response Relationship Drug Mutagenicity Tests fungi food and beverages Ethylenes Rats chemistry Agrotechnological Research Institute Non polar Fuel Oils Mutagens |
| Zdroj: | Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis 391 (1997) Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 391, 117-125 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/s0165-1218(97)00028-1 |
| Popis: | A series of cyclopenta-fused polynuclear aromatic hydrocarbons (PAH) were tested for mutagenicity in a bacterial forward mutation assay based on resistance to 8-azaguanine (8-AG) in Salmonella typhimurium TM677 in the absence of Aroclor-induced rat liver postmitochondrial supernatant (PMS). All of the aceanthrylenes tested were mutagenic in the absence of PMS, whereas none of the acephenanthrylenes were active. The following mutagenic potency series expressed as the minimum detectable mutagen concentration (MDMC) in nmol/ml was obtained: aceanthrylene (AA) (5.5); cyclopent[ h,i ]aceanthrylene (CPAA) (18.2); 6-methylaceanthrylene (6-MeAA) (112); 1,2,6,7-tetrahydrocyclopent[ h,i ]aceanthrylene (THCPAA) (166); 1,2-dihydroaceanthrylene (DHAA) (298). Saturation of the cyclopenta rings or methylation at the 6-position of AA reduced, but did not eliminate, mutagenicity measured in the absence of PMS. AA was unusual because it was approximately 4-fold more mutagenic in the absence of PMS than in its presence. The other aceanthrylenes tested were 1.3–10.7 times more mutagenic in the presence of PMS than in its absence to give an MDMC potency series of: CPAA (3.8); 6-MeAA (10.5); AA (19.9); THCPAA (52.9); DHAA (229). Approximately 20% of the PMS-independent mutagenicity in a combustion sample from ethylene burned under fuel-rich conditions was found in a fraction containing only non-polar, 4–7 ring PAHs, widely attributed to be mutagenic only in the presence of PMS. None of this mutagenicity could be attributed to aceanthrylenes, thus other non-polar PAHs appear to possess significant PMS-independent mutagenicity as well. |
| Databáze: | OpenAIRE |
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