Clonal insulinoma cell line that stably maintains correct glucose responsiveness
Autor: | David Knaack, Deborah M. Fiore, Manju Surana, David Fusco-DeMane, Norman Fleischer, Margarita Leiser, Orion D. Hegre, Megan E. Laurance, Shimon Efrat |
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Rok vydání: | 1994 |
Předmět: |
medicine.medical_specialty
Monosaccharide Transport Proteins medicine.medical_treatment Antigens Polyomavirus Transforming Endocrinology Diabetes and Metabolism Molecular Sequence Data Mice Transgenic Biology Islets of Langerhans Mice Internal medicine Hexokinase Glucokinase medicine Tumor Cells Cultured Internal Medicine Animals Insulin RNA Messenger Promoter Regions Genetic Insulinoma Cell Line Transformed Glucose Transporter Type 2 Glucose Transporter Type 1 Base Sequence Glucose transporter medicine.disease Molecular biology Clone Cells Transplantation Pancreatic Neoplasms Microscopy Electron Endocrinology Glucose Phenotype Cell culture biology.protein GLUT2 GLUT1 |
Zdroj: | Diabetes. 43:1413-1417 |
ISSN: | 0012-1797 |
Popis: | A number of pancreatic β-tumor cell (β TC) lines have been derived from insulinomas arising in transgenic mice expressing the SV40 T antigen gene under control of the insulin promoter. Some of these lines secrete insulin in response to physiological glucose concentrations. However, this phenotype is unstable. After propagation in culture, these nonclonal lines become responsive to subphysiological glucose levels and/or manifest reduced insulin release. Here we report the use of soft-agar cloning to isolate single-cell clones from a β TC line, which give rise to sublines that maintain correct glucose responsiveness and high insulin production and secretion for > 55 passages (over a year) in culture. One of these clonal lines, denoted β TC6-F7, was characterized in detail. β TC6-F7 cells expressed high glucokinase and low hexokinase activity, similarly to normal islets. In addition, they expressed mRNA for the GLUT2 glucose transporter isotype and no detectable GLUT1 mRNA, as is characteristic of normal β-cells. These results demonstrate that transformed β-cells can maintain a highly differentiated phenotype during prolonged propagation in culture, which has implications for the development of continuous β-cell lines for transplantation therapy of diabetes. |
Databáze: | OpenAIRE |
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