Complete genome of Staphylococcus aureus Tager 104 provides evidence of its relation to modern systemic hospital-acquired strains
| Autor: | Paul E. Bock, Matthias Nahrendorf, Andrew D. Brannen, Scott Monsma, Mark R. Liles, Michael J. Lodes, Peter Panizzi, Richard Davis, David A. Mead, Mohammad J. Hossain |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Proteome 030106 microbiology Virulence Microbial Sensitivity Tests Biology Staphylococcal infections medicine.disease_cause Genome Evolution Molecular 03 medical and health sciences Genetics medicine Animals Humans Genomic library Phylogeny Gene Library Cross Infection Staphylococcal Infections medicine.disease 3. Good health Bacterial Typing Techniques Mice Inbred C57BL 030104 developmental biology Multilocus sequence typing Female DNA microarray Mobile genetic elements Sequence Alignment Genome Bacterial Software Biotechnology Multilocus Sequence Typing Research Article |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of these MGE, as well as single nucleotide polymorphisms, is of high clinical and microbiological importance. To understand the evolution of these dangerous pathogens, it is paramount to define reference strains that may predate MGE acquisition. One such candidate is S. aureus Tager 104, a previously uncharacterized strain isolated from a patient with impetigo in 1947. Results We show here that S. aureus Tager 104 can survive in the bloodstream and infect naïve organs. We also demonstrate a procedure to construct and validate the assembly of S. aureus genomes, using Tager 104 as a proof-of-concept. In so doing, we bridged confounding gap regions that limited our initial attempts to close this 2.82 Mb genome, through integration of data from Illumina Nextera paired-end, PacBio RS, and Lucigen NxSeq mate-pair libraries. Furthermore, we provide independent confirmation of our segmental arrangement of the Tager 104 genome by the sole use of Lucigen NxSeq libraries filled by paired-end MiSeq reads and alignment with SPAdes software. Genomic analysis of Tager 104 revealed limited MGE, and a νSaβ island configuration that is reminiscent of other hospital acquired S. aureus genomes. Conclusions Tager 104 represents an early-branching ancestor of certain hospital-acquired strains. Combined with its earlier isolation date and limited content of MGE, Tager 104 can serve as a viable reference for future comparative genome studies. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2433-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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