Towards artificial metallonucleases for gene therapy: recent advances and new perspectives
| Autor: | Béla Gyurcsik, Anikó Czene |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pharmacology
Genetics Models Molecular Dna targeting Endodeoxyribonucleases DNA Repair Genetic enhancement Protein engineering DNA Genetic Therapy Biology Protein Engineering chemistry.chemical_compound chemistry Metals Drug Discovery Gene Targeting State of art Molecular Medicine Animals Humans Gene |
| Zdroj: | Future medicinal chemistry. 3(15) |
| ISSN: | 1756-8927 |
| Popis: | The process of DNA targeting or repair of mutated genes within the cell, induced by specifically positioned double-strand cleavage of DNA near the mutated sequence, can be applied for gene therapy of monogenic diseases. For this purpose, highly specific artificial metallonucleases are developed. They are expected to be important future tools of modern genetics. The present state of art and strategies of research are summarized, including protein engineering and artificial ‘chemical’ nucleases. From the results, we learn about the basic role of the metal ions and the various ligands, and about the DNA binding and cleavage mechanism. The results collected provide useful guidance for engineering highly controlled enzymes for use in gene therapy. |
| Databáze: | OpenAIRE |
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