Characterisation of a Marine Bacterium Vibrio Brasiliensis T33 Producing N-acyl Homoserine Lactone Quorum Sensing Molecules
| Autor: | Kok-Gan Chan, Wai-Fong Yin, Wen-Si Tan, Tan-Guan-Sheng Adrian, Nur Izzati Mohamad, Nina Yusrina Muhamad Yunos, Pui-Wan Tan |
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| Rok vydání: | 2014 |
| Předmět: |
Oceans and Seas
quorum sensing (QS) Chromobacterium violaceum CV026 Acyl-Butyrolactones Biology biosensor lcsh:Chemical technology Biochemistry Article Analytical Chemistry Microbiology chemistry.chemical_compound Marine bacteriophage Species Specificity 16S rDNA Seawater lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Vibrio Biofilm Quorum Sensing food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification 16S ribosomal RNA Atomic and Molecular Physics and Optics marine water-borne N-acyl homoserine lactone (AHL) N-hexanoyl-l-homoserine lactone (C6-HSL) N-(3-oxo-decanoyl)-l-homoserine lactone (3-oxo-C10 HSL) Vibrio brasiliensis Quorum sensing N-Acyl homoserine lactone chemistry Chromobacterium violaceum Bacteria |
| Zdroj: | Sensors, Vol 14, Iss 7, Pp 12104-12113 (2014) Sensors (Basel, Switzerland) Sensors; Volume 14; Issue 7; Pages: 12104-12113 |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s140712104 |
| Popis: | N-acylhomoserine lactones (AHL) plays roles as signal molecules in quorum sensing (QS) in most Gram-negative bacteria. QS regulates various physiological activities in relation with population density and concentration of signal molecules. With the aim of isolating marine water-borne bacteria that possess QS properties, we report here the preliminary screening of marine bacteria for AHL production using Chromobacterium violaceum CV026 as the AHL biosensor. Strain T33 was isolated based on preliminary AHL screening and further identified by using 16S rDNA sequence analysis as a member of the genus Vibrio closely related to Vibrio brasiliensis. The isolated Vibrio sp. strain T33 was confirmed to produce N-hexanoyl-l-homoserine lactone (C6-HSL) and N-(3-oxodecanoyl)-l-homoserine lactone (3-oxo-C10 HSL) through high resolution tandem mass spectrometry analysis. We demonstrated that this isolate formed biofilms which could be inhibited by catechin. To the best of our knowledge, this is the first report that documents the production of these AHLs by Vibrio brasiliensis strain T33. |
| Databáze: | OpenAIRE |
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