Characterization of the cattle serum antibody responses against TEM beta-lactamase and the nonimmunogenic Escherichia coli heat-stable enterotoxin (STaI)
| Autor: | Paola Sandra Mercuri, Nadia Ruth, Ingrid Antoniotti, Béatrice Robert, Astrid Zervosen, Frédéric Schynts, Alfred Collard, Moreno Galleni, Claude Saegerman |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Immunology Bacterial Toxins Cattle Diseases Enterotoxin medicine.disease_cause digestive system Microbiology Immunoglobulin G beta-Lactamases Enterotoxins Mice Antigen medicine Escherichia coli Immunology and Allergy Heat-stable enterotoxin Animals Escherichia coli Infections Mice Inbred BALB C Vaccines Synthetic biology Toxin Escherichia coli Vaccines Escherichia coli Proteins General Medicine Antibodies Bacterial Infectious Diseases Humoral immunity biology.protein Cattle Immunization Antibody |
| Zdroj: | FEMS immunology and medical microbiology. 54(3) |
| ISSN: | 0928-8244 |
| Popis: | In order to test the use of a subunit recombinant vaccine for its capacity to induce antibodies against the nonimmunogenic heat-stable enterotoxin STa from Escherichia coli and the TEM-1 beta-lactamase, cattle were immunized with a hybrid protein created by insertion of the STa sequence at position 197 of the TEM-1 beta-lactamase. Specific anti-STa IgG and IgG1 antibodies were detected at low levels, while no IgG2 antibodies were detected. In contrast, high levels of the different anti-TEM IgG subtypes were detected in cattle sera. In addition, beta-lactamase activity was inhibited by the sera. The presence of antibodies against STa and TEM-1 beta-lactamase was assessed in sera from 366 cattle taken from the field. No significant level of IgGs against the toxin or the TEM-1 was detected. A comparison of the antibody level between the immunized and the nonimmunized animals clearly demonstrated that STa was not able to induce a significant level of antibodies in the vaccinated animals. In contrast, a strong antibody response against TEM-1 beta-lactamase was demonstrated. |
| Databáze: | OpenAIRE |
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