Serum Antibodies Positivity to 12 Helicobacter pylori Virulence Antigens in Patients with Benign or Malignant Gastroduodenal Diseases – Cross-sectional Study

Autor: Slavko Gašparov, Miroslava Katičić, Tajana Filipec Kanižaj, Branko Kolarić, Anna Mrzljak, Vladimir Presečki, Vesna Čolić Cvrlje
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Male
Pathology
Gastroenterology
Endoscopy
Gastrointestinal
Duodenal Neoplasms
Odds Ratio
Stomach cancer
Antrum
Gastrointestinal Neoplasms
biology
Incidence
Stomach
Intestinal metaplasia
General Medicine
Middle Aged
Clinical Science
Prognosis
medicine.anatomical_structure
H. pylori
gastritis
virulence antigens
CagA
VacA
p 19
p 30
p 33
Gastritis
Female
medicine.symptom
Adult
Peptic Ulcer
medicine.medical_specialty
Risk Assessment
Sensitivity and Specificity
Helicobacter Infections
Age Distribution
Stomach Neoplasms
Internal medicine
Confidence Intervals
medicine
Humans
Sex Distribution
Aged
Antigens
Bacterial
Helicobacter pylori
biology.organism_classification
medicine.disease
bacterial infections and mycoses
digestive system diseases
Cross-Sectional Studies
Duodenal Ulcer
Multivariate Analysis
bacteria
Bacterial antigen
Zdroj: Croatian Medical Journal
Volume 50
Issue 2
ISSN: 1332-8166
0353-9504
Popis: Aim: To investigate the association of gastric histological and endoscopic findings in patients with Helicobacter pylori (H. pylori), according to presence of seropositivity to 12 bacterial virulence antigens. ----- Methods: This is a cross-sectional single-center study of 360 consecutive outpatients referred in the period of one year to upper gastrointestinal endoscopy because of dyspeptic complaints. Patients sera were tested by Western blot method to determine the presence of serum antibodies to bacterial virulence antigens – p120 (CagA – cytotoxin-associated antigen), p95 (VacA – vacuolating cytotoxin), p67 (FSH – flagellar sheath protein), p66 (UreB – urease enzyme heavy subunit), p57 (HSP homologue – heath shock protein homologue), p54 (flagellin), p33, p30 (OMP – outer membrane protein), p29 (UreA – urease enzyme light subunit), p26, p19, and p17. Upper gastrointestinal endoscopy was performed, endoscopic diagnosis recorded, and 4 mucosal biopsy samples were obtained and assessed according to Updated Sydney protocol. ----- Results: The sera of 207 patients were analyzed. Thirty patients had gastric adenocarcinoma, 126 peptic ulcers, and 51 normal finding. p120 (CagA) seropositivity was significantly more often present in patients with higher activity grade in the antrum (P = 0.025), p30 in patients with greater inflammation in the antrum (P = 0.025) and the corpus (P = 0.010), p33 in patients with greater inflammation in the corpus (P = 0.050), and p19 (OMP) in patients with lower intestinal metaplasia grades in the corpus (P = 0.025). Seroreactivity to all other bacterial proteins showed no association with the histological status of the stomach mucosa. Except for the seropositivity to protein p95 (VacA), which was more often present in patients with duodenal ulcer (P = 0.006), there was no difference in seroreactivity to other bacterial proteins and upper gastrointestinal endoscopic findings. ----- Conclusions: p120 (CagA), p33, p 30 (OMP), and p19 (OMP) seropositivity was more often present in patients with higher grades of the histological parameters of gastritis and seropositivity to protein p95 (VacA) with endoscopic presence of duodenal ulcer. Histological parameters of gastritis are more associated with bacterial virulence than endoscopic findings.
Databáze: OpenAIRE
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