RGD-targeted paramagnetic liposomes for early detection of tumor: In vitro and in vivo studies
| Autor: | Yongmei Ding, Lixia Ju, Wei Li, Caicun Zhou, Linghua Yan, Wei Zhou, Yin Song, Liang Tang, Bo Su, Heyan Li, Shuyan Meng, Yinmin Zhao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Gadolinium DTPA
Biodistribution Lung Neoplasms Transplantation Heterologous Mice Nude Adenocarcinoma In Vitro Techniques Sensitivity and Specificity Mice chemistry.chemical_compound Drug Delivery Systems In vivo Cell Line Tumor Animals Humans Medicine Radiology Nuclear Medicine and imaging Lipid bilayer Nuclear Magnetic Resonance Biomolecular Analysis of Variance Mice Inbred BALB C Liposome business.industry Lipopeptide General Medicine In vitro Transplantation chemistry Liposomes Immunology Biophysics Molecular imaging business Oligopeptides |
| Zdroj: | European Journal of Radiology. 80:598-606 |
| ISSN: | 0720-048X |
| DOI: | 10.1016/j.ejrad.2011.01.051 |
| Popis: | Magnetic resonance molecular imaging has emerged as a potential approach for tumor diagnosis in the last few decades. This approach consists of the delivery of MR contrast agents to the tumor by specific targeted carriers. For this purpose, a lipopeptide was constructed by using a cyclic RGD peptide headgroup coupled to palmitic acid anchors via a KGG tripeptide spacer. Targeted paramagnetic liposomes were then prepared by the incorporation of RGD-coupled-lipopeptides into lipid bilayers for specific bounding to tumor. In vitro, study demonstrated that RGD-targeted liposomes exhibited a better binding affinity to targeted cells than non-targeted liposomes. MR imaging of mice bearing A549 tumors with the RGD-targeted paramagnetic liposomes also resulted in a greater signal enhancement of tumor compared to non-targeted liposomes and pure contrast agents groups. In addition, biodistribution study also showed specific tumor targeting of RGD-targeted paramagnetic liposomes in vivo. Therefore, RGD-targeted paramagnetic liposomes prepared in the present study may be a more promising method for early tumor diagnosis. |
| Databáze: | OpenAIRE |
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