Opioidergic and adrenergic modulation of formalin-evoked spinal c-fos mRNA expression and nocifensive behavior in the rat
| Autor: | Mervyn Maze, Shigehito Sawamura, M. F. Davies, M Fujinaga, Wade S. Kingery, N Belanger |
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| Rok vydání: | 1999 |
| Předmět: |
Agonist
Male medicine.medical_specialty medicine.drug_class Narcotic Antagonists Pain Biology c-Fos Rats Sprague-Dawley Opioid receptor Receptors Adrenergic alpha-2 Internal medicine Formaldehyde medicine Animals RNA Messenger Pain Measurement Pharmacology Opioidergic Inflammation Analgesics Atipamezole Adrenergic alpha-2 Receptor Antagonists Rats Endocrinology Nociception Spinal Cord Receptors Opioid Morphine biology.protein Opiate Inflammation Mediators Proto-Oncogene Proteins c-fos medicine.drug |
| Zdroj: | European journal of pharmacology. 379(2-3) |
| ISSN: | 0014-2999 |
| Popis: | Fos protein expression has been used to reflect neuronal activation in pain processing pathways although analgesics may uncouple behavioral and Fos responses. We determine whether formalin-induced spinal c-fos mRNA expression (Northern blotting) correlates with nocifensive behavior following pretreatment with morphine, the alpha2-adrenoceptor agonist dexmedetomidine, or their respective antagonists naloxone and atipamezole. Both opiate and alpha2-adrenoceptor agonists reduced formalin-induced c-fos gene transcription and nocifensive behavior via their cognate receptors. Unexpectedly, blockade of either the opiate or alpha2-adrenergic receptors, alone, caused an increase in formalin-evoked c-fos mRNA; while blocking the opiate receptor had no effect on formalin-induced behavior, alpha2-adrenoceptor block had an analgesic effect, indicating discordance between c-fos message transcription and nocifensive behavior. We concluded that the formalin-induced spinal c-fos signal was a poor predictor of the behavioral response to pharmacological manipulation of pain processing pathways. |
| Databáze: | OpenAIRE |
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