Antimicrobial activities of ethanol and butanol fractions of white rose petal extract
| Autor: | Jae Kwon Choi, Jong Bae Kim, Yeseul Cha, Yoon Bok Lee, Nam Soo Han, Ehn-Kyoung Choi, Sa-Hyun Kim, Dongsun Park, Kyungha Shin, Young Jin Choi, Haiyu Guo, Seong Soo Joo, Yun-Bae Kim |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Butanols Colony Count Microbial Flowers Microbial Sensitivity Tests Toxicology Rosa Microbiology law.invention Helicobacter Infections 03 medical and health sciences Propionibacterium acnes chemistry.chemical_compound 0302 clinical medicine Anti-Infective Agents law Gastric mucosa medicine Animals Ethanol Plants Medicinal biology Dose-Response Relationship Drug Helicobacter pylori Inoculation General Medicine biology.organism_classification Antimicrobial Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry Gastric Mucosa 030220 oncology & carcinogenesis Solvents Phytotherapy Bacteria |
| Zdroj: | Regulatory toxicology and pharmacology : RTP. 76 |
| ISSN: | 1096-0295 |
| Popis: | White rose (Rosa hybrida) petals were extracted with ethanol (EtOH) or butanol (BuOH), and tested for their antimicrobial activities against two species of Gram-positive bacteria, six species of Gram-negative bacteria, and two species of fungi. On in vitro antimicrobial assays, Helicobacter pylori and Propionibacterium acnes were highly susceptible to white rose petal extract (WRPE)-EtOH and WRPE-BuOH, leading to minimal inhibitory concentrations of 100 and 10 μg/mL for H. pylori and 400 and 40 μg/mL for P. acnes, respectively. In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric inoculation (1 × 10(8) CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying 75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg. The CLO test results were confirmed by bacterial identification. WRPE-EtOH and WRPE-BuOH inhibited the growth of various bacteria and fungi, and in particular, they effectively killed H. pylori and eliminated the bacteria from the mouse stomach. The results indicate that WRPE-EtOH and WRPE-BuOH could be good candidates for the elimination of H. pylori. |
| Databáze: | OpenAIRE |
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