Wheat embryo ribonucleates. XIV. Mass isolation of mRNA from wheat germ and comparison of its translational capacity with that of mRNA from imbibing wheat embryos
| Autor: | Byron G. Lane, Theresa D. Kennedy, A. C. Cuming |
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| Rok vydání: | 1979 |
| Předmět: |
Peptide Biosynthesis
Reticulocytes Sucrose Fractionation Biology chemistry.chemical_compound Reticulocyte medicine Animals RNA Messenger Polyacrylamide gel electrophoresis Triticum Messenger RNA Cell-Free System Molecular mass food and beverages RNA Embryo General Medicine Plants Molecular biology Molecular Weight medicine.anatomical_structure chemistry Biochemistry Protein Biosynthesis Rabbits Poly A |
| Zdroj: | Canadian Journal of Biochemistry. 57:1170-1175 |
| ISSN: | 0008-4018 |
| DOI: | 10.1139/o79-151 |
| Popis: | Commercially milled wheat germ is shown to be a convenient source material for facile recovery of mass (milligram) quantities of highly purified poly(A)-rich RNA. This poly(A)-rich RNA is efficiently translated in a nuclease-treated extract of rabbit reticulocytes. By sucrose density gradient fractionation of bulk poly(A)-rich RNA from wheat germ, it has been possible to show that there is a direct relationship between the molecular weights of the polypeptide products of cell-free synthesis and the molecular weights of the wheat mRNA molecules which program their synthesis. As assessed by SDS – polyacrylamide gel electrophoresis, the same array of polypeptides is synthesized when nuclease-treated reticulocyte extract is programmed by poly(A)-rich RNA from either commercially supplied or laboratory-prepared wheat embryos. Significantly, there are gross quantitative if not qualitative differences between the translational capacities of poly(A)-rich RNA from dry and imbibing wheat embryos, and the possible importance of these differences for interpreting a changing pattern of polypeptide synthesis in imbibing wheat embryos is the subject of a brief discussion. |
| Databáze: | OpenAIRE |
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