A panel of intestinal differentiation markers (CDX2, GPA33, and LI-cadherin) identifies gastric cancer patients with favourable prognosis
| Autor: | Nair Lopes, Merete Bjørnslett, Luísa Pereira, Raquel Almeida, Eric P. Bennett, Patrícia Mesquita, Leonor David, Bruno Cavadas, Rita Pinto, Rosa Gomes, Christian H. Bergsland, Jarle Bruun, André Filipe Vieira, Ragnhild A. Lothe |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Cancer Research 03 medical and health sciences 0302 clinical medicine Surgical oncology Stomach Neoplasms medicine Biomarkers Tumor Humans Clinical significance CDX2 Transcription Factor CDX2 Aged Retrospective Studies GPA33 Tissue microarray Membrane Glycoproteins Cadherin business.industry Gastroenterology Cancer Cell Differentiation General Medicine medicine.disease Cadherins Prognosis Antigens Differentiation digestive system diseases Intestines Survival Rate Oncology 030220 oncology & carcinogenesis Cancer research Immunohistochemistry 030211 gastroenterology & hepatology Female business Follow-Up Studies |
| Zdroj: | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. 23(5) |
| ISSN: | 1436-3305 |
| Popis: | Gastric cancer is the fifth most common cancer and the third cause of global cancer mortality. CDX2 is an intestinal differentiation marker with prognostic value in gastric cancer and transcriptionally regulates the expression of glycoprotein A33 (GPA33) and liver intestine cadherin (LI-cadherin). This study evaluated the clinical significance of the combined expression of CDX2 and its targets GPA33 and LI-cadherin in gastric cancer by fluorescence-based multiplex immunohistochemistry together with digital image analysis and chromogenic immunohistochemistry in 329 gastric cancer samples arranged in tissue microarrays. Additionally, publicly available RNA-seq expression data from 354 gastric cancer samples from the TCGA database were used to validate the immunohistochemistry results. Expression of the three markers (CDX2, GPA33, and LI-cadherin) was strongly correlated, defining an intestinal differentiation panel. Low or negative protein expression of the intestinal differentiation panel identified patients with particularly poor overall survival, irrespective of the methodology used, and was validated in the independent series at the RNA-seq level. Expression of the intestinal differentiation panel (CDX2, GPA33, and LI-cadherin) defines a set of biomarkers with a strong biological rationale and favourable impact for prognostication of gastric cancer patients. |
| Databáze: | OpenAIRE |
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