Voltage-gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87-MG cells
| Autor: | Lin Chen, Ming‑Shan Pi, Bao Miao Ma, Chao Ying Li, Qin Ru, Qi Xiong, Xiang Tian, Kai Yue |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Quinidine
Cancer Research Cell Apoptosis Biology Cell Line Tumor Potassium Channel Blockers medicine Humans Channel blocker RNA Messenger Cell Proliferation Voltage-gated ion channel Cell growth Cell Cycle Glioma Cell cycle Potassium channel Cell biology Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure Oncology Potassium Channels Voltage-Gated Cancer research medicine.drug |
| Zdroj: | International Journal of Oncology. 46:833-840 |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2014.2777 |
| Popis: | Accumulating evidence has proved that potassium channels (K+ channels) are involved in regulating cell proliferation, cell cycle progression and apoptosis of tumor cells. However, the precise cellular mechanisms are still unknown. In the present study, we investigated the effect and mechanisms of quinidine, a commonly used voltage-gated K+ channel blocker, on cell proliferation and apoptosis of human glioma U87-MG cells. We found that quinidine significantly inhibited the proliferation of U87-MG cells and induced apoptosis in a dose-dependent manner. The results of caspase colorimetric assay showed that the mitochondrial pathway was the main mode involved in the quinidine-induced apoptotic process. Furthermore, the concentration range of quinidine, which inhibited voltage-gated K+ channel currents in electrophysiological assay, was consistent with that of quinidine inhibiting cell proliferation and inducing cell apoptosis. In U87-MG cells treated with quinidine (100 µmol/l), 11 of 2,042 human microRNAs (miRNAs) were upregulated and 16 were downregulated as detected with the miRNA array analysis. The upregulation of miR-149-3p and downregulation of miR-424-5p by quinidine treatment were further verified by using quantitative real-time PCR. In addition, using miRNA target prediction program, putative target genes related to cell proliferation and apoptosis for two differentially expressed miRNAs were predicted. Taken together, these data suggested that the anti-proliferative and pro-apoptosis effect of voltage-gated K+ channel blocker quinidine in human glioma cells was mediated at least partly through regulating expression of miRNAs, and provided further support for the mechanisms of voltage-gated K+ channels in mediating cell proliferation and apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|