Monoclonal antibodies reactive in routinely processed tissue sections of malignant lymphoma, with emphasis on T-cell lymphomas
Autor: | Cedith M. Copenhaver, Robin McDaid, John G. Strickler, David G. Buck, Lawrence M. Weiss, Roger A. Warnke, J. Bindl |
---|---|
Rok vydání: | 1987 |
Předmět: |
Pathology
medicine.medical_specialty Lymphoma medicine.drug_class T cell T-Lymphocytes Monoclonal antibody Stain Pathology and Forensic Medicine Malignant lymphoma Immunoenzyme Techniques Antigen immune system diseases Antibody Specificity hemic and lymphatic diseases medicine Humans B-Lymphocytes biology business.industry Antibodies Monoclonal medicine.disease Hodgkin Disease medicine.anatomical_structure Tissue sections biology.protein Antibody business |
Zdroj: | Human pathology. 18(8) |
ISSN: | 0046-8177 |
Popis: | The immunoreactivity of eight monoclonal antibodies was evaluated on 45 routinely processed lymphomas (22 T-cell lymphomas, 11 B-cell lymphomas, and 12 cases of Hodgkin's disease). Two antibodies reactive with leukocyte common (T200) antigens (PD7/26 and 2B11) stained most of the B- and T-cell lymphomas but did not stain the Reed-Sternberg cells and variants in Hodgkin's disease. Two antibodies known to stain B cells (LN-1 and LN-2) reacted with some of the B-cell lymphomas, but LN-2 also reacted with the neoplastic cells in six of 22 T-cell lymphomas and with the Reed-Sternberg variants in eight of 12 cases of Hodgkin's disease. The granulocyte antibody anti-Leu M1 reacted with most cases of Hodgkin's disease but also reacted with two of 11 B-cell non-Hodgkin's lymphomas. An antibody to epithelial membrane antigen (anti-EMA) stained some cases of T-cell lymphoma, B-cell lymphoma, and Hodgkin's disease. Leu 7 was expressed in one T-cell lymphoma and in one case of Hodgkin's disease. A novel antibody reactive with T cells (L60) stained all cases of T-cell lymphoma but also stained some cases of B-cell lymphoma and one case of Hodgkin's disease. We conclude that none of these antibodies, when used alone on routinely fixed paraffin-embedded material, is completely sensitive and specific for T-cell lymphoma, B-cell lymphoma, or Hodgkin's disease. However, a panel of antibodies is useful in distinguishing Hodgkin's disease from non-Hodgkin's lymphoma and in suggesting the B- or T-cell phenotype of non-Hodgkin's lymphomas. |
Databáze: | OpenAIRE |
Externí odkaz: |