Design and analysis of a squamous cell carcinoma in vitro model system
| Autor: | Samantha Nolan, Katja Schenke-Layland, Eva Brauchle, Sibylle Thude, Hannah Johannsen |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Skin Neoplasms Biophysics Human skin Bioengineering Biology Organ culture Spectrum Analysis Raman Models Biological Epithelium Biomaterials Dermis Cell Line Tumor medicine Humans In vitro test Child Skin Artificial Principal Component Analysis integumentary system Epidermis (botany) Staining and Labeling Infant Immunohistochemistry In vitro medicine.anatomical_structure Cell culture Mechanics of Materials Child Preschool Carcinoma Squamous Cell Ceramics and Composites Co-culture Epidermis Keratinocyte |
| Zdroj: | Biomaterials. 34(30):7401-7407 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2013.06.016 |
| Popis: | Tissue-engineered skin equivalents based on primary isolated fibroblasts and keratinocytes have been shown to be useful tools for functional in vitro tests, including toxicological screenings and drug development. In this study, a commercially available squamous cell carcinoma (SCC) cell line SCC-25 was introduced into epidermal and full-thickness skin equivalents to generate human-based disease-in-a-dish model systems. Interestingly, when cultured either in the epidermis or dermis of full-thickness skin equivalents, SCC-25 cells formed hyper-keratinized tumor cell nests, a phenomenon that is frequently seen in the skin of patients afflicted with SCC. Raman spectroscopy was employed for the label-free cell phenotype characterization within the engineered skin equivalents and revealed the presence of differential protein patterns in keratinocytes and SCC-25 cells. To conclude, the here presented SSC disease-in-a-dish approaches offer the unique opportunity to model SSC in human skin in vitro, which will allow further insight into SSC disease progression, and the development of therapeutic strategies. |
| Databáze: | OpenAIRE |
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