Detection of waterborne norovirus genogroup I strains using an improved real time RT-PCR assay
| Autor: | Han-Gil Cho, Weon-Hwa Jheong, Myung-Jin Lee, S Y Paik, Su-Kyoung Mun, Mi-Hye Yoon, Sung-Geun Lee, Po-Hyun Park |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Genotype Reverse Transcriptase Polymerase Chain Reaction Norovirus 030106 microbiology Outbreak General Medicine Acute gastroenteritis Biology Virology Microbiology 03 medical and health sciences Medical microbiology Real-time polymerase chain reaction stomatognathic system Norovirus Genogroup I medicine Water Microbiology Feces |
| Zdroj: | Archives of Virology. 162:3389-3396 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-017-3505-z |
| Popis: | Noroviruses (NoVs) are the major global source of acute gastroenteritis (AGE) outbreaks. To detect NoVs, real-time reverse transcription-quantitative PCR (RT-qPCR) assays have been widely employed since the first decade of the 21st century. We developed a redesigned probe, JJV1PM, for RT-qPCR assay detection of NoV genogroup (G) I strains. The new RT-qPCR assay using the JJV1PM-probe showed broader strain reactivity for 10 NoV GI genotypes, while the old method, using the JJV1PT-probe assay, detected only 7 NoV GI genotypes in a validation panel using human fecal specimens. The improved RT-qPCR assay was also successfully applied to water samples. The JJV1PM-probe assay identified 7 NoV GI genotypes, whereas the JJV1PT-probe assay detected only 2 NoV GI genotypes from water samples. Notably, groundwater-borne NoV GI strains detected by the improved JJV1PM-probe assay were associated with groundwater-borne AGE outbreaks in South Korea. The results of this study underscore the importance of the evaluation of RT-qPCR assays using recently circulating NoV strains prior to field application. |
| Databáze: | OpenAIRE |
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