Intracellular stored calcium plays a minor role in Gq-coupled receptor-mediated contraction in rat airway smooth muscle
| Autor: | Ting Kang Nee, Mei Kee Lee, Yvonne Mbaki, Kayatri Govindaraju |
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| Rok vydání: | 2018 |
| Předmět: |
Agonist
medicine.medical_specialty Carbachol medicine.drug_class lcsh:Biotechnology chemistry.chemical_element trachea Calcium lcsh:TP248.13-248.65 Internal medicine medicine Extracellular Receptor lcsh:QH301-705.5 Phenylephrine lcsh:R5-920 Smooth muscle tissue biology Chemistry stored and extracellular calcium phenylephrine carbachol aorta Endocrinology medicine.anatomical_structure lcsh:Biology (General) Gq alpha subunit biology.protein lcsh:Medicine (General) medicine.drug |
| Zdroj: | Life Sciences, Medicine and Biomedicine, Vol 2, Iss 2 (2018) |
| ISSN: | 2600-7207 |
| Popis: | The general notion of activation of Gq-protein coupled receptors involves the mobilisation of stored and extracellular calcium and leads to smooth muscle tissue contraction. The aim of this study was to investigate the involvement of calcium mediated contractions in vascular and airway smooth muscles. Using the standard organ bath procedures, aortic and tracheal rings were obtained from 6 to 8 week-old male Sprague Dawley rats. To activate the Gq protein receptors, phenylephrine (PE), an α1-adrenoceptor agonist, and carbachol, a M3 cholinoceptor agonist, was added to baths containing the aortic and tracheal rings, respectively. The maximum response (Emax) to PE was reduced from 158.8 ± 11.8% (n=6) to 62.5 ± 12.4 % (n=8) upon removal of extracellular calcium in Krebs-Ringer solution. Maximal response to PE was also suppressed in the presence of nifedipine, a L-type Ca2+ channel inhibitor, (70.3 ± 11 %, n=8) and SKF96365, a canonical transient receptor potential channel inhibitor, (26.7 ± 13.2 %, n=5) when the influx of extracellular calcium was blocked. Removal of stored calcium also attenuated the PE contraction (p0.05). From these observations, we conclude that the role of stored and extracellular calcium in Gq protein activation is not the same across different type of smooth muscle tissues. |
| Databáze: | OpenAIRE |
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