An in Vitro Biosynthesis of Sesquiterpenes Starting from Acetic Acid
Autor: | Julia Nowack, Frank Schulz, Michael Dirkmann |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Patchoulol Stereochemistry Farnesyl pyrophosphate Mevalonic Acid Naphthols Mevalonic acid Biochemistry Cofactor Terpene 03 medical and health sciences chemistry.chemical_compound Acetic acid Polyisoprenyl Phosphates Biosynthesis Molecular Biology Acetic Acid Bacteria biology Organic Chemistry Fungi Plants Archaea Terpenoid Enzymes 030104 developmental biology chemistry Cyclization Biocatalysis biology.protein Molecular Medicine Sesquiterpenes |
Zdroj: | ChemBioChem. 19:2146-2151 |
ISSN: | 1439-4227 |
DOI: | 10.1002/cbic.201800128 |
Popis: | The enzymatic synthesis of terpenes was investigated by using a cascade based on the mevalonic acid pathway. Suitable enzymes from all kingdoms of life were identified and combined to give rise to geosmin and patchoulol as representative compounds. The pathway was studied in three separate segments, which were subsequently combined in a ten-step cascade plus added cofactor regeneration systems. The cascade delivers farnesyl pyrophosphate with >40 % conversion and cyclises it to sesquiterpenes with >90 % conversion. |
Databáze: | OpenAIRE |
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