Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil

Autor: Vanessa Riesz Salgado, Leonardo José Richtzenhain, Paulo Eduardo Brandão, Alessandra Marnie Martins Gomes de Castro, João Pessoa Araújo, Taís F. Cruz, Karen L Ferrari, Tatiana M. Kanashiro
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp)
Rok vydání: 2012
Předmět:
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Acta Veterinaria Scandinavica
Acta Veterinaria Scandinavica, Vol 54, Iss 1, p 29 (2012)
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Made available in DSpace on 2013-08-28T14:11:54Z (GMT). No. of bitstreams: 1 WOS000308347400001.pdf: 289762 bytes, checksum: 0f5e97b302207de7f389c0243831aac1 (MD5) Made available in DSpace on 2013-09-30T18:38:33Z (GMT). No. of bitstreams: 2 WOS000308347400001.pdf: 289762 bytes, checksum: 0f5e97b302207de7f389c0243831aac1 (MD5) WOS000308347400001.pdf.txt: 18106 bytes, checksum: cbfd619522c19ec4a88b75a5d22cb622 (MD5) Previous issue date: 2012-05-03 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:51:11Z No. of bitstreams: 2 WOS000308347400001.pdf: 289762 bytes, checksum: 0f5e97b302207de7f389c0243831aac1 (MD5) WOS000308347400001.pdf.txt: 18106 bytes, checksum: cbfd619522c19ec4a88b75a5d22cb622 (MD5) Made available in DSpace on 2014-05-20T13:51:11Z (GMT). No. of bitstreams: 2 WOS000308347400001.pdf: 289762 bytes, checksum: 0f5e97b302207de7f389c0243831aac1 (MD5) WOS000308347400001.pdf.txt: 18106 bytes, checksum: cbfd619522c19ec4a88b75a5d22cb622 (MD5) Previous issue date: 2012-05-03 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Porcine circovirus type 2 (PCV2) has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-infections with other infectious organisms.Findings: Samples of 168 aborted foetuses or mummified foetuses from five farrow-to-finish swine farms known to be infected with PCV2 and located in the State of São Paulo were tested for PCV2 by polymerase chain reaction (PCR). Positive samples were additionally tested for porcine parvovirus (PPV), Leptospira spp. and Brucella spp. by PCR. PCV2 was detected in 18 of the samples (10.7%). PPV, Brucella spp. and Leptospira spp were found in 2, 10 and 0 cases, respectively. Eleven PCV2 strains were sequenced and determined to be either genotype 2a (n = 1) or 2b (n = 10).Conclusions: The findings indicate that the frequency of PCV2 infections in aborted porcine foetuses from the State of São Paulo is rather low (10.7%) and that co-infection with other pathogens is common and may be involved in PCV2 associated reproductive failure. No repeatable, characteristic amino acid motifs for regions of the PCV2 capsid protein seemed to be associated with abortion in sows. Univ São Paulo, Dept Prevent Vet Med & Anim Hlth, Coll Vet Med, BR-05508270 São Paulo, Brazil São Paulo State Univ, Dept Immunol & Microbiol, Inst Biosci, Coll Vet Med, BR-186180 Botucatu, SP, Brazil São Paulo State Univ, Dept Immunol & Microbiol, Inst Biosci, Coll Vet Med, BR-186180 Botucatu, SP, Brazil FAPESP: 07/50003-5
Databáze: OpenAIRE
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