The effect of perfusion culture on proliferation and differentiation of human mesenchymal stem cells on biocorrodible bone replacement material
| Autor: | Jana Farack, Gisela Standke, Berthold Nies, Stefan Glorius, Hartmut Worch, Peter Quadbeck, Cornelia Wolf-Brandstetter, Dieter Scharnweber |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
Mechanical Engineering Metallurgy Mesenchymal stem cell chemistry.chemical_element hydroxyapatite Adhesion bioactive metal foams Calcium Condensed Matter Physics medicine.disease_cause human mesenchymal stem cells (hMSCs) Perfusion Culture chemistry Mechanics of Materials Cell culture In vivo Biophysics medicine General Materials Science Brushite perfusion culture Oxidative stress brushite |
| Popis: | Biocorrodible iron foams were coated with different calcium phosphate phases (CPP) to obtain a bioactive surface and controlled degradation. Further adhesion, proliferation and differentiation of SaOs-2 and human mesenchymal stem cells were investigated under both static and dynamic culture conditions. Hydroxyapatite (HA; [Ca 10 (PO 4 ) 6 OH 2 ]) coated foams released 500 μg/g iron per day for Dulbecco's modified eagle medium (DMEM) and 250 μg/g iron per day for McCoys, the unmodified reference 1000 μg/g iron per day for DMEM and 500 μg/g iron per day for McCoys, while no corrosion could be detected on brushite (CaHPO 4 ) coated foams. Using a perfusion culture system with conditions closer to the in vivo situation, cells proliferated and differentiated on iron foams coated with either brushite or HA while in static cell culture cells could proliferate only on Fe-brushite. We conclude that the degradation behaviour of biocorrodible iron foams can be varied by different calcium phosphate coatings, offering opportunities for design of novel bone implants. Further studies will focus on the influence of different modifications of iron foams on the expression of oxidative stress enzymes. Additional information about in vivo reactions and remodelling behaviour are expected from testing in implantation studies. |
| Databáze: | OpenAIRE |
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