Proteomics characterization of exosome cargo
| Autor: | J. Matthew Luther, Kristie L. Rose, Kevin L. Schey |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Proteome Exosome biogenesis Annexins Molecular Sequence Data Computational biology Biology Bioinformatics Exosomes Exosome General Biochemistry Genetics and Molecular Biology Mass Spectrometry Article Antigens CD Humans Amino Acid Sequence Molecular Biology Endosomal Sorting Complexes Required for Transport Biological Transport ANTIGENS CD Response to treatment Microvesicles DNA-Binding Proteins Bibliometrics Posttranslational modification Protein Processing Post-Translational Biomarkers Transcription Factors |
| Zdroj: | Methods (San Diego, Calif.). 87 |
| ISSN: | 1095-9130 |
| Popis: | Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis. |
| Databáze: | OpenAIRE |
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