Preliminary Assessment of Visible, Near-Infrared, and Short-Wavelength–Infrared Spectroscopy with a Portable Instrument for the Detection of Staphylococcus aureus Biofilms on Surfaces
| Autor: | Pablo Rodríguez-Gonzálvez, Ana Rodríguez, Juanjo Peón, Carmen Recondo, Cristina Allende-Prieto, Pilar García, Beatriz Martínez, Lucía Fernández, Diana Gutiérrez |
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| Přispěvatelé: | Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Principado de Asturias, Ministerio de Ciencia, Innovación y Universidades (España), Universidad de Oviedo, Fernández, Lucía [0000-0002-7383-7595], Gutiérrez, Diana [0000-0002-0473-1447], Martínez Fernández, Beatriz [0000-0001-7692-1963], García Suárez, María Pilar [0000-0003-1213-8165], Rodríguez González, Ana [0000-0002-1577-9905], Fernández, Lucía, Gutiérrez, Diana, Martínez Fernández, Beatriz, García Suárez, María Pilar, Rodríguez González, Ana |
| Rok vydání: | 2019 |
| Předmět: |
Food industry
Infrared Infrared spectroscopy medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences medicine Spectroscopy 030304 developmental biology 0303 health sciences Chromatography biology Chemistry Visible near infrared 010401 analytical chemistry Biofilm biology.organism_classification 0104 chemical sciences Detection Wavelength Staphylococcus aureus Biofilms Bacteria Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0362-028X |
| Popis: | Bacterial biofilms constitute a major source of sanitary problems and economic losses in the food industry. Indeed, biofilm removal may require intense mechanical cleaning procedures or very high concentrations of disinfectants or both, which can be damaging to the environment and human health. This study assessed the efficacy of a technique based on spectroscopy in the visible, near-infrared, and short-wavelength infrared range for the quick detection of biofilms formed on polystyrene by the pathogenic bacterium Staphylococcus aureus. To do that, biofilms corresponding to three S. aureus strains, which differed in biofilm-forming ability and composition of the extracellular matrix, were allowed to develop for 5 or 24 h, representing an active formation stage and mature biofilms, respectively. Spectral analysis of the samples, corresponding to three biological replicates of each condition, was then performed by using a portable device. The results of these experiments showed that partial least-squares discriminant analysis of the spectral profile could discriminate between surfaces containing attached bacterial biomass and noninoculated ones. In this model, the two first principal components accounted for 39 and 19% of the variance and the estimated error rate stabilized after four components. Cross-validation accuracy of this assessment was 100%. This work lays the foundation for subsequent development of a spectroscopy-based protocol that allows biofilm detection on food industrial surfaces. This work was funded by the following grants: Program of Science, Technology and Innovation 2013–2017 (AGL2015-65673-R), Ministry of Economy, Industry and Competitiveness, Spain (EU Animal Health and Welfare [ANIHWA] ERA-Net; BLAAT ID: 67/PCIN-2017-001), European Regional Development Fund (ERDF) EU funds, Principado de Asturias, Spain (Proyecto Intramural CSIC 201770E016, GRUPIN14-139 and IDI/2018/000119), Ministerio de Economía y Competitividad, Spain (BIO2017-88147-R), and Programa de Apoyo y Promoción de la Investigación, University of Oviedo, Spain (PAPI 2018) |
| Databáze: | OpenAIRE |
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