Subcutaneous transplantation of bone marrow derived stem cells in macroencapsulation device for treating diabetic rats; clinically transplantable site
| Autor: | Sherry M. Khater, Sawsan M. El-Halawani, Mahmoud M. Gabr, Mahmoud M. Zakaria, Mohamed A. Ghoneim, Mohamed El-Far, Ayman F. Refaie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Cell biology medicine.medical_treatment Stem cells Biochemistry Article 03 medical and health sciences Endocrinology 0302 clinical medicine Diabetes mellitus medicine Subcutaneous transplantation lcsh:Social sciences (General) lcsh:Science (General) Multidisciplinary business.industry Insulin Mesenchymal stem cell Diabetes Immunosuppression medicine.disease Transplantation Biological sciences 030104 developmental biology medicine.anatomical_structure Regenerative medicine Pancreatic islet transplantation lcsh:H1-99 Bone marrow Stem cell business 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 5, Pp e03914-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Background/aim Diabetes mellitus (DM) is a serious, chronic and epidemic disease. Its effective therapy with exogenous insulin places an overwhelming burden on the patient's lifestyle. Moreover, pancreatic islet transplantation is limited by the scarceness of donors and the need for chronic immunosuppression. Cell-based therapy is considered an alternative source of insulin-producing cells (IPCs); encapsulating such cellular grafts in immunoisolating devices would protect the graft from immune attack without the need for immunosuppression. Herein, we investigate the ability of TheraCyte capsule as an immunoisolating device to promote the maturation of differentiated rat bone marrow derived mesenchymal stem cells (BM-MSCs), transplanted subcutaneously to treat diabetic rats in comparison with intratesticular transplantation. Main methods Rat BM-MSC were differentiated into IPCs, and either encapsulated in TheraCyte capsules for subcutaneous transplantation or transplanted intratesticular into diabetic rats. Serum insulin, C-peptide & blood glucose levels of transplanted animals were monitored. Retrieved cells were further characterized by immunofluorescence staining and gene expression analysis. Key findings Differentiated rat BM-MSC were able to produce insulin in vitro, ameliorate hyperglycemia in vivo and survive for 6 months post transplantation. Transplanted cells induced higher levels of insulin and C-peptide, lower levels of blood glucose in the cured animals of both experimental groups. Gene expression revealed a further in vivo maturation of the implanted cells. Significance These data suggest that TheraCyte encapsulation of allogeneic differentiated stem cells are capable of reversing hyperglycemia, which holds a great promise as a new cell based, clinically applicable therapies for diabetes. Stem cells, Diabetes, Subcutaneous transplantation; Biological sciences; Cell biology; Biochemistry; Endocrinology; Regenerative medicine. |
| Databáze: | OpenAIRE |
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