Intra-arterial transplantation of stem cells in large animals as a minimally-invasive strategy for the treatment of disseminated neurodegeneration
| Autor: | Joanna Sanford, Joanna Głodek, Michal Zawadzki, Miroslaw Janowski, Lukasz Kalkowski, Joanna Kwiatkowska, Zbigniew Adamiak, Piotr Holak, Dominika Golubczyk, Piotr Walczak, Kamila Milewska, Izabela Malysz-Cymborska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Pathology Swine Science Fluorescent Antibody Technique Disease Mesenchymal Stem Cell Transplantation Article Dogs medicine.artery medicine Distribution (pharmacology) Animals Minimally Invasive Surgical Procedures Regeneration and repair in the nervous system Stem cells in the nervous system Multidisciplinary business.industry Neurodegeneration Mesenchymal stem cell Disease Management Mesenchymal Stem Cells Neurodegenerative Diseases medicine.disease Immunohistochemistry Magnetic Resonance Imaging Transplantation Disease Models Animal Surgery Computer-Assisted Middle cerebral artery Diseases of the nervous system Medicine Histopathology Stem cell business Neuroscience Stem Cell Transplantation |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Stem cell transplantation proved promising in animal models of neurological diseases; however, in conditions with disseminated pathology such as ALS, delivery of cells and their broad distribution is challenging. To address this problem, we explored intra-arterial (IA) delivery route, of stem cells. The goal of this study was to investigate the feasibility and safety of MRI-guided transplantation of glial restricted precursors (GRPs) and mesenchymal stem cells (MSCs) in dogs suffering from ALS-like disease, degenerative myelopathy (DM). Canine GRP transplantation in dogs resulted in rather poor retention in the brain, so MSCs were used in subsequent experiments. To evaluate the safety of MSC intraarterial transplantation, naïve pigs (n = 3) were used as a pre-treatment control before transplantation in dogs. Cells were labeled with iron oxide nanoparticles. For IA transplantation a 1.2-French microcatheter was advanced into the middle cerebral artery under roadmap guidance. Then, the cells were transplanted under real-time MRI with the acquisition of dynamic T2*-weighted images. The procedure in pigs has proven to be safe and histopathology has demonstrated the successful and predictable placement of transplanted porcine MSCs. Transplantation of canine MSCs in DM dogs resulted in their accumulation in the brain. Interventional and follow-up MRI proved the procedure was feasible and safe. Analysis of gene expression after transplantation revealed a reduction of inflammatory factors, which may indicate a promising therapeutic strategy in the treatment of neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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