Importance of prostanoids in calcium chelator-induced airway constriction
| Autor: | Mark C. Liu, K. S. Lindeman, Carol A. Hirshman, N. Downey, W. C. Hubbard, Anne Kagey-Sobotka |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Physiology
chemistry.chemical_element Prostaglandin Calcium Pharmacology Methylprednisolone chemistry.chemical_compound Dogs Physiology (medical) medicine Animals Edetic Acid Chelating Agents Meclofenamic Acid Bronchial Spasm medicine.diagnostic_test Airway Resistance Prostanoid respiratory system Acetylcholine Thromboxane B2 Bronchoalveolar lavage chemistry Anesthesia Prostaglandins Meclofenamate Sodium lipids (amino acids peptides and proteins) Bronchoconstriction medicine.symptom Bronchoalveolar Lavage Fluid Histamine |
| Zdroj: | Journal of Applied Physiology. 75:1110-1116 |
| ISSN: | 1522-1601 8750-7587 |
| Popis: | To elucidate the mechanism of calcium chelator-induced airway constriction, we examined the relationship between prostanoids and histamine in bronchoalveolar lavage (BAL) fluid and the magnitude of airway constriction in peripheral airways of anesthetized Basenji-Greyhound dogs. A wedged bronchoscope technique was used to measure collateral system resistance (Rcs) before and after aerosol challenges. Sublobar segments were challenged either with acetylcholine or with Na2EDTA in the presence or absence of meclofenamate sodium (3 mg/kg iv) or methylprednisolone (2 mg.kg-1.day-1). After measurements of Rcs, BAL was performed, and the fluid was analyzed for prostanoids with the use of gas chromatography-mass spectrometry. Sublobar segments challenged with Na2EDTA showed increased concentrations of prostaglandin (PG) D2 but no increases in PGE2, PGF2 alpha, 9 alpha,11 beta-PGF2, 6-keto-PGF1 alpha, thromboxane B2, or histamine. There was a strong relationship (r = 0.84, P = 0.005) between changes in Rcs after Na2EDTA and concentrations of PGD2 in BAL fluid. Acetylcholine, which increased Rcs to a similar degree as Na2EDTA did, produced no significant increase in prostanoid concentrations. Changes in Rcs after Na2EDTA and concentrations of PGD2 were reduced in the presence of meclofenamate or methylprednisolone. These data support the idea that the mechanism of calcium chelator-induced bronchoconstriction involves the release of bronchoconstricting prostanoids. |
| Databáze: | OpenAIRE |
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