Mycobacterium branderi from Both a Hand Infection and a Case of Pulmonary Disease
| Autor: | Christine Turenne, Michelle J. Alfa, Godfrey K. M. Harding, Louise Thibert, Amin Kabani, Joyce Wolfe |
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| Rok vydání: | 2000 |
| Předmět: |
Lung Diseases
Microbiology (medical) Mycobacterium branderi medicine.drug_class Antibiotics Human pathogen Microbial Sensitivity Tests Case Reports DNA Ribosomal Mycobacterium Microbiology Ciprofloxacin Clarithromycin RNA Ribosomal 16S Trimethoprim Sulfamethoxazole Drug Combination medicine Humans Ribosomal DNA Chromatography High Pressure Liquid Mycobacterium Infections biology Skin Diseases Bacterial Middle Aged Ribosomal RNA Hand biology.organism_classification Molecular diagnostics 16S ribosomal RNA Mycolic Acids Drug Therapy Combination Female Bacteria |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Mycobacterium branderi , a potential human pathogen first characterized in 1995, has been isolated from respiratory tract specimens. We report here a case in which M. branderi was the only organism isolated upon culture from a hand infection. This isolate, along with a second isolate from a bronchial specimen, was subjected to conventional identification tests for mycobacterial species. Further analysis by high-performance liquid chromatography (HPLC) of mycolic acids and 16S rRNA gene sequencing was performed, and the antibiotic susceptibility profile was determined for both strains. Biochemical tests and the HPLC pattern were consistent with that of M. branderi and M. celatum , which are very similar. The 16S rRNA gene sequence of both strains corresponded to that of M. branderi and enabled us to confidently differentiate this organism from other closely related species such as M. celatum . This contributes to a further understanding of the status of this species as a potential human pathogen as well as illustrating the need for molecular diagnostics as a complementary method for the identification of rare mycobacterial species. |
| Databáze: | OpenAIRE |
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