Strain-Dependent Effects of Acute Alcohol on Synaptic Vesicle Recycling and Post-Tetanic Potentiation in Medial Glutamate Inputs to the Mouse Basolateral Amygdala
| Autor: | Dominic A Gioia, Brian A. McCool |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Refractory Period Electrophysiological Glutamic Acid Medicine (miscellaneous) Stimulation Pharmacology Toxicology Synaptic Transmission Article Exocytosis Mice 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine Species Specificity mental disorders medicine Animals Synaptic vesicle recycling Mice Inbred BALB C Ethanol Post-tetanic potentiation Basolateral Nuclear Complex Chemistry Glutamate receptor Long-term potentiation Electric Stimulation Mice Inbred C57BL Psychiatry and Mental health 030104 developmental biology medicine.anatomical_structure Mice Inbred DBA Synaptic Vesicles Tetanic stimulation Neuroscience 030217 neurology & neurosurgery Synaptic vesicle priming Basolateral amygdala |
| Zdroj: | Alcoholism: Clinical and Experimental Research. 41:735-746 |
| ISSN: | 0145-6008 |
| DOI: | 10.1111/acer.13343 |
| Popis: | BACKGROUND Inbred mouse strains are differentially sensitive to the acute effects of ethanol (EtOH) and are useful tools for examining how unique genomes differentially affect alcohol-related behaviors and physiology. DBA/2J mice have been shown to be sensitive to the acute anxiolytic effects of alcohol as well as the anxiogenic effects of withdrawal from chronic alcohol exposure, while B6 mice are resistant to both. Considering that the basolateral amygdala (BLA) is an important brain region for the acute and chronic effects of EtOH on fear and anxiety related behaviors, we hypothesized that there would be strain-dependent differences in the acute effects of EtOH in BLA slices. METHODS We utilized patch clamp electrophysiology in BLA coronal slices from 4 inbred mouse strains (A/J, BALBcJ, C57BL/6J, and DBA/2J) to examine how genetic background influences acute EtOH effects on synaptic vesicle recycling and post-tetanic potentiation (PTP) in response to low (2 Hz)- and high (40 Hz)-frequency stimulation. RESULTS We found that EtOH inhibited synaptic vesicle recycling in a strain- and stimulation frequency-dependent manner. Vesicle recycling in DBA/2J and BALBcJ cells was inhibited by acute EtOH during both low- and high-frequency stimulation, while recycling measured from A/J cells was sensitive only during high-frequency stimulation. Recycling at C57BL/6J synapses was insensitive to EtOH regardless of stimulation frequency. We additionally found that cells from DBA/2J and BALBcJ mice were sensitive to EtOH-mediated inhibition of PTP. CONCLUSIONS Acute EtOH application inhibited vesicle recycling and PTP at glutamatergic synapses in both a strain- and frequency-dependent fashion. Several presynaptic proteins that contribute to synaptic vesicle priming in addition to PTP have been implicated in alcohol-related behaviors, including Munc13, Munc18, and RIM proteins, making them potential candidates for the molecular mechanism controlling these effects. |
| Databáze: | OpenAIRE |
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