Long non‐coding RNA ADAMTS9‐AS1 suppresses colorectal cancer by inhibiting the Wnt/β‐catenin signalling pathway and is a potential diagnostic biomarker
Autor: | Helen Binang, Mi Qi, Juan Li, Ning Li, Qing Wang, Peilong Li, Yingjie Chen, Yan Xie, Chuanxin Wang, Yanlei Wang, Haiting Mao, Yunshan Wang, Lili Wang, Lutao Du |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
endocrine system Epithelial-Mesenchymal Transition Colorectal cancer Carcinogenesis Down-Regulation colorectal cancer exosomes Biology medicine.disease_cause 03 medical and health sciences 0302 clinical medicine In vivo Cell Line Tumor medicine Transcriptional regulation Biomarkers Tumor Humans Neoplasm Metastasis Gene Wnt Signaling Pathway Cell Proliferation Cell Nucleus Cell growth Gene Expression Profiling EMT Cell Biology Original Articles medicine.disease Microvesicles Long non-coding RNA LncRNA Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine ADAMTS9‐AS1 Original Article RNA Long Noncoding Colorectal Neoplasms |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Long non‐coding RNAs (lncRNAs) have come out as critical molecular regulators of human tumorigenesis. In this study, we sought to identify and functionally characterize lncRNAs as potential mediators of colorectal cancer progression. We screened and identified a novel lncRNA, ADAMTS9‐AS1, which was significantly decreased in colorectal cancer tissues and was correlated with clinical outcome of patients according to The Cancer Genome Atlas (TCGA) database. In addition, ADAMTS9‐AS1 regulated cell proliferation and migration both in vitro and in vivo. Bioinformatics analysis revealed that overexpression of lncRNA‐ADAMTS9‐AS1 preferentially affected genes that were linked to proliferation and migration. Mechanistically, we found that ADAMTS9‐AS1 obviously suppressed β‐catenin, suggesting that Wnt signalling pathway participates in ADAMTS9‐AS1‐mediated gene transcriptional regulation in the suppression of colorectal tumorigenesis. Finally, we found that exosomal ADAMTS9‐AS1 could serve as a diagnostic biomarker for colorectal cancer with AUC = 0.835 and 95% confidence interval = 0.777‐0.911. Our data demonstrated that ADAMTS9‐AS1 might play important roles in colorectal cancer by suppressing oncogenesis. Targeting ADAMTS9‐AS1 may have potential clinical applications in colorectal cancer prognosis and treatment as an ideal therapeutic target. Finally, exosomal lncRNA‐ADAMTS9‐AS1 is a promising, novel diagnostic biomarker for colorectal cancer. |
Databáze: | OpenAIRE |
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