Capillary electrophoretic analysis of the antibiotic vancomycin in innovative microparticles and in commercial formulations
| Autor: | Vittorio Zecchi, Barbara Luppi, Roberto Mandrioli, Maria Augusta Raggi, Alessandro Musenga, Salvatore Fanali |
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| Přispěvatelé: | Musenga A., Mandrioli R., Zecchi V., Luppi B., Fanali S., Raggi M.A. |
| Rok vydání: | 2006 |
| Předmět: |
Capillary action
medicine.drug_class Chemistry Pharmaceutical Clinical Biochemistry Pharmaceutical Science Capsules Glycopeptide antibiotic Dosage form GLYCOPEPTIDE ANTIBIOTIC Analytical Chemistry Capillary electrophoresis Drug Discovery medicine Spectroscopy Antibacterial agent Chromatography Aqueous solution Chemistry Extraction (chemistry) Electrophoresis Capillary CAPILLARY ZONE ELECTROPHORESIS Hydrogen-Ion Concentration VANCOMYCIN Controlled release Anti-Bacterial Agents CONTROLLED VANCOMYCIN RELEASE INNOVATIVE MICROPARTICLES |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 42:32-38 |
| ISSN: | 0731-7085 |
| Popis: | A new fast capillary electrophoretic method has been developed for the analysis of the glycopeptide antibiotic vancomycin in formulations. An electrophoretic run is completed within 3.0 min; fused silica capillaries (100 μm i.d., 8.5 cm effective length and 48.5 cm total length) and a background electrolyte consisting of 12.5 mM, pH 2.5 phosphate buffer are used. The applied voltage is −20.0 kV; samples are injected by pressure (30 mbar × 3 s) at the anodic end of the capillary. The method was successfully applied to innovative controlled release microparticles consisting of a coated albumin core containing vancomycin. A simple procedure has been developed to obtain complete vancomycin extraction from microparticles using a 5% (w/v) sodium dodecyl sulphate aqueous solution. The method has been validated in terms of linearity, precision and accuracy. Good linearity was found in the 0.25–5.00 μg/mL range. Satisfactory precision was obtained, with relative standard deviation values always lower than 3.9%; accuracy was satisfactory, with recovery values between 97.8 and 102.2%. The method is also suitable for vancomycin determination in commercial capsules. |
| Databáze: | OpenAIRE |
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