The Role of the DIF Motif of the DnaJ (Hsp40) Co-chaperone in the Regulation of the DnaK (Hsp70) Chaperone Cycle
| Autor: | Gordana Cogelja Cajo, Pierre Genevaux, Françoise Schwager, Costa Georgopoulos, William L. Kelley, B. Erin Horne |
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| Rok vydání: | 2006 |
| Předmět: |
Glycine/chemistry
Phenylalanine Amino Acid Motifs Molecular Sequence Data Glycine HSP40 Heat-Shock Proteins/ chemistry/metabolism Molecular Chaperones/chemistry medicine.disease_cause Biochemistry Escherichia coli Molecular chaperone Dna J DnaK DnaJ Chaperone Cycle Conserved sequence Escherichia coli Proteins/ chemistry/metabolism Adenosine Triphosphate ATP hydrolysis medicine Animals Humans HSP70 Heat-Shock Proteins Amino Acid Sequence Molecular Biology Peptide sequence Conserved Sequence ddc:616 chemistry.chemical_classification Genetics biology Escherichia coli Proteins Hydrolysis Cell Biology HSP40 Heat-Shock Proteins Amino acid Cell biology Co-chaperone Complementation HSP70 Heat-Shock Proteins/ chemistry/metabolism Adenosine Triphosphate/chemistry Phenylalanine/chemistry chemistry Chaperone (protein) Escherichia coli/metabolism biology.protein Molecular Chaperones |
| Zdroj: | Journal of Biological Chemistry, Vol. 281, No 18 (2006) pp. 12436-12444 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m511192200 |
| Popis: | To perform effectively as a molecular chaperone, DnaK (Hsp70) necessitates the assistance of its DnaJ (Hsp40) co-chaperone partner, which efficiently stimulates its intrinsically weak ATPase activity and facilitates its interaction with polypeptide substrates. In this study, we address the function of the conserved glycine- and phenylalanine-rich (G/F-rich) region of the Escherichia coli DnaJ in the DnaK chaperone cycle. We show that the G/F-rich region is critical for DnaJ co-chaperone functions in vivo and that despite a significant degree of sequence conservation among the G/F-rich regions of Hsp40 homologs from bacteria, yeast, or humans, functional complementation in the context of the E. coli DnaJ is limited. Furthermore, we found that the deletion of the whole G/F-rich region is mirrored by mutations in the conserved Asp-Ile/Val-Phe (DIF) motif contained in this region. Further genetic and biochemical analyses revealed that this amino acid triplet plays a critical role in regulation of the DnaK chaperone cycle, possibly by modulating a crucial step subsequent to DnaK-mediated ATP hydrolysis. |
| Databáze: | OpenAIRE |
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