Rat liver epithelial cell cultures in a serum-free medium: Primary cultures and derived cell lines expressing differentiated functions
| Autor: | Prudent Padieu, Martine Chessebeuf |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Plant Science
Biology Dexamethasone Cell Line Bile Acids and Salts chemistry.chemical_compound Biosynthesis medicine Animals Bovine serum albumin Cells Cultured Progesterone Tyrosine Transaminase Primary (chemistry) Cholic acid Cell Differentiation Epithelial Cells Metabolism Epithelium Culture Media Rats medicine.anatomical_structure Liver Biochemistry chemistry Cell culture biology.protein Developmental biology Cell Division Biotechnology |
| Zdroj: | In Vitro. 20:780-795 |
| ISSN: | 1475-2689 0073-5655 |
| Popis: | Rat liver epithelial cells explanted in a serum-free medium (SFM) composed of Ham's F10 basal medium plus free fatty acids adsorbed on bovine albumin gave successful rise to primary cultures and then to long-term cell lines that expressed liver functions; induction of L-tyrosine aminotransferase by glucocorticoids, hepatic pattern of progesterone metabolism, and biosynthesis of murine primary bile acids; chenodeoxycholic and cholic acid common to higher vertebrates and alpha-muricholic acid specific of the rat bile. |
| Databáze: | OpenAIRE |
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