Synthesis and dose interval dependent hepatotoxicity evaluation of intravenously administered polyethylene glycol-8000 coated ultra-small superparamagnetic iron oxide nanoparticle on Wistar rats
| Autor: | Balan Rajan, Thiruvengadam Devaki, Shanmugam Sathish, Subramanian Balakumar |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Health Toxicology and Mutagenesis Aspartate transaminase Spleen Pharmacology Kidney Toxicology Polyethylene Glycols Microscopy Electron Transmission Drug tolerance Spectroscopy Fourier Transform Infrared medicine Animals Aspartate Aminotransferases Rats Wistar Lung Liver injury Glutathione Peroxidase biology Superoxide Dismutase Chemistry Magnetic Phenomena Brain Alanine Transaminase Heart gamma-Glutamyltransferase General Medicine Alkaline Phosphatase Catalase medicine.disease Ferrosoferric Oxide Enzyme assay Glutathione Reductase medicine.anatomical_structure Liver Biochemistry Alanine transaminase biology.protein Nanoparticles Alkaline phosphatase Administration Intravenous |
| Zdroj: | Environmental Toxicology and Pharmacology. 39:727-735 |
| ISSN: | 1382-6689 |
| Popis: | Superparamagnetic iron oxide nanoparticles are being used in medical imaging, drug delivery, cancer therapy, and so on. However, there is a direct need to identify any nanotoxicity associated with these nanoparticles. However uncommon, drug-induced liver injury (DILI) is a major health concern that challenges pharmaceutical industry and drug regulatory agencies alike. In this study we have synthesized and evaluated the dose interval dependent hepatotoxicity of polyethylene glycol-8000 coated ultra-small superparamagnetic iron oxide nanoparticles (PUSPIOs). To assess the hepatotoxicity of intravenously injected PUSPIOs, alterations in basic clinical parameters, hematological parameters, hemolysis assay, serum levels of liver marker enzymes, serum and liver lipid peroxidation (LPO) levels, enzymatic antioxidant levels, and finally histology of liver, kidney, spleen, lung, brain, and heart tissues were studied in control and experimental Wistar rat groups over a 30-day period. The results of our study showed a significant increase in the aspartate transaminase (AST) enzyme activity at a dose of 10 mg/kg b.w. PUSPIOs twice a week. Besides, alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (γGT) enzyme activity showed a slender increase when compared with control experimental groups. A significant increase in the serum and liver LPO levels at a dose of 10 mg/kg b.w. PUSPIOs twice a week was also observed. Histological analyses of liver, kidney, spleen, lung, brain and heart tissue samples showed no obvious uncharacteristic changes. In conclusion, PUSPIOs were found to posses excellent biocompatibility and Wistar rats showed much better drug tolerance to the dose of 10 mg/kg b.w. per week than the dose of 10 mg/kg b.w. twice a week for the period of 30 days. |
| Databáze: | OpenAIRE |
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