Anti-Peptide Antibodies Directed Against Omega-Gliadins for the Detection of Sequences from Bread and Durum Wheats
| Autor: | S. Denery-Papini, J. C. Autran, M. F. Samson |
|---|---|
| Přispěvatelé: | UR 0724 Unité de recherche Biochimie et technologie des protéines, Institut National de la Recherche Agronomique (INRA)-Transformation des Produits Végétaux (T.P.V.)-Unité de recherche Biochimie et technologie des protéines (NANT LBTP), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
[SDV]Life Sciences [q-bio] Immunology Peptide 01 natural sciences Botany Food science Durum Wheats ComputingMilieux_MISCELLANEOUS Antiserum chemistry.chemical_classification biology Chemistry digestive oral and skin physiology 010401 analytical chemistry nutritional and metabolic diseases food and beverages digestive system diseases 0104 chemical sciences Polyclonal antibodies biology.protein Antibody Gliadin Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Food and Agricultural Immunology Food and Agricultural Immunology, Taylor & Francis, 2000, 12, pp.67-75. ⟨10.1080/09540100099634⟩ |
| ISSN: | 1465-3443 0954-0105 |
| DOI: | 10.1080/09540100099634 |
| Popis: | This paper presents the characterisation of polyclonal antibodies directed against two different N-terminal sequences of ω-gliadins. Both antisera recognised specifically the corresponding omega-gliadin fractions but showed different reactivities against ω-gliadins extracted from bread and durum wheats. Antibodies directed against the 'SRLL' type (ω5 type) recognised several ω-gliadin components in bread and durum wheat extracts whereas antibodies directed against the 'AREL' type (ω2 type) reacted specifically with an ω-gliadin component from bread wheat. The narrow specificity of this antiserum made it potentially interesting for the detection of bread wheat additions in durum wheat pasta. Moreover, the reactivity of this antiserum was not modified by an increase in drying temperature of pasta. |
| Databáze: | OpenAIRE |
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