Use of leukocytes as treatment for endometritis in mares experimentally infected with Streptococcus equi subsp. zooepidemicus
| Autor: | Gabriella Möller, Marisa Ribeiro de Itapema Cardoso, Rodrigo Costa Mattos, Cristina Rodrigues Trein, Holm Zerbe, Adriana Pires Neves, Andrea Keller, Maria Inês Mascarenhas Jobim, E Klug, Luis Fernando Fiori Castilho, Wolfgang Leibold, Ricardo Macedo Gregory |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
animal diseases Biology medicine.disease_cause Microbiology Endocrinology Food Animals Estrus Cytology Streptococcal Infections medicine Leukocytes Animals Streptococcus equi Horses reproductive and urinary physiology Estrous cycle Cross-Over Studies urogenital system Streptococcus Inoculation Interleukin-8 General Medicine medicine.disease biology.organism_classification Immunity Innate Streptococcus equi subsp. zooepidemicus Immunology Streptococcus zooepidemicus Animal Science and Zoology Female Horse Diseases Endometritis Disease Susceptibility Bacteria |
| Zdroj: | Animal reproduction science. 97(3-4) |
| ISSN: | 0378-4320 |
| Popis: | This study compared four treatments for bacterial endometritis in mares experimentally infected with Streptococcus zooepidemicus . Twenty-five mares were used, 20 resistant and five susceptible to endometritis. Mares would be in estrus when infected. Twenty-four hours after inoculation, clinical, bacteriological and cytological examinations were performed and repeated until the first occurrence: negative cytology and no Streptococcus growth or the seventh day post-infection. All mares showed clinical signs of endometritis and were assigned to one of the following treatments: (1) intrauterine infusion of fresh leukocytes; (2) intrauterine infusion of frozen-thawed leukocytes; (3) intrauterine infusion of lysed leukocytes; (4) intrauterine infusion of recombinant human interleukin-8 (rhIL-8); (5) control. Mares were submitted to all treatments, with at least a 14-day interval between treatments in a Latin square design. Treatment did not affect ( P = 0.121) time needed for resistant mares to eliminate bacteria. Time needed for elimination of bacteria was similar in susceptible mares treated with fresh and frozen leukocytes ( P = 0.333). Susceptible mares treated with frozen leukocytes also did not differ from those treated with lysed leukocytes ( P = 0.227) for time to eliminate bacteria, but were significantly different ( P > 0.02) from those treated with rhIL-8 and control. In resistant mares, physical clearance ability was probably the responsible for bacterial elimination. Intrauterine infusions in susceptible mares with viable or lysed leukocytes associated or not to opsonizing factors, reduced the time to elimination of bacteria. Infusions with bactericidal effect (functional neutrophils and granules) was likely effective and responsible for the more rapid elimination of bacteria in susceptible mares. |
| Databáze: | OpenAIRE |
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