Multilocus Sequence Typing of Strains of Bacterial Spot of Lettuce Collected in the United States
| Autor: | Richard N. Raid, Ken Pernezny, Pamela D. Roberts, Jeffrey B. Jones, Carolee T. Bull, Erica M. Goss, Joubert Fayette |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genotype Plant Science Xanthomonas campestris 01 natural sciences California 03 medical and health sciences Bacterial Proteins Genetic variation Gene Phylogeny Ohio Plant Diseases Genetics Genetic diversity Virulence biology Haplotype Genetic Variation Lettuce biology.organism_classification Bacterial Typing Techniques Housekeeping gene Plant Leaves 030104 developmental biology Haplotypes Florida Multilocus sequence typing Agronomy and Crop Science Multilocus Sequence Typing 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 106:1262-1269 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-11-15-0302-r |
| Popis: | Studies on genetic diversity and recombination in bacterial pathogens are providing a better understanding of the mechanisms shaping bacterial diversity, which can affect disease control. Xanthomonas campestris pv. vitians, causal agent of bacterial leaf spot of lettuce, is a threat to the worldwide lettuce industry. We examined the genetic variation within a sample of 83 strains from California, Florida, and Ohio using multilocus sequence typing of six housekeeping genes, totaling 2.7 kb. Additionally, polymorphism in two virulence-related genes, hrpB2 and a putative glycosyl hydrolase, were examined. Based on housekeeping genes, we found three genetic groups of strains that were all able to induce the disease. These included strains collected from weeds and irrigation water that had haplotypes identical to strains from diseased lettuce. High linkage disequilibrium across the sequenced loci indicates that the pathogen is predominantly clonal but recombination has contributed to the observed sequence variation. Although there was significant genetic variation in X. campestris pv. vitians within and among sampled states, identical haplotypes were observed across all three states. This finding suggests that seedborne inoculum may contribute to the diversity of X. campestris pv. vitians in the United States. Knowledge of the genetic structure of the pathogen may be used for developing resistant lettuce varieties. |
| Databáze: | OpenAIRE |
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