Rat lung antioxidant enzyme activities and their specific proteins during hyperoxia
| Autor: | J. B. Snyder, Russell A. Prough, J B Warshaw, Kathleen A. Kennedy, L. S. Crouch |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Aging Antioxidant Physiology medicine.medical_treatment Glutathione reductase Blotting Western Dehydrogenase Biology Glucosephosphate Dehydrogenase chemistry.chemical_compound Organ Culture Techniques In vivo Physiology (medical) Internal medicine medicine Glucose-6-phosphate dehydrogenase Animals Lung Hyperoxia Staining and Labeling Rats Inbred Strains Glutathione Enzyme assay Rats Oxygen Endocrinology Glutathione Reductase chemistry Biochemistry Liver biology.protein medicine.symptom |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 8750-7587 |
| Popis: | The hyperoxia-induced increases in the activity of lung glucose-6-phosphate dehydrogenase (G-6-P) and glutathione reductase (GR) after exposure of rats to greater than 97% O2 for 6 days were accompanied by equivalent increases in the amount of the respective immunoreactive proteins. Hyperoxia also increased lung glutathione (GSH) + oxidized glutathione (GSSG) content and the magnitude of this hyperoxic response of increased GSH + GSSG, G-6-P, and GR (maximal 1.3- to 1.8-fold) declined as a function of age during the first 3 wk of life. Fetal rat lung explants cultured 4 days in 95% O2 showed increased G-6-P and GR activity and increased levels of the specific proteins 1.5-fold those of explants at 2 days of culture. We conclude that the hyperoxic response of increased rat lung G-6-P and GR activity in vivo and in vitro involves not just alteration of enzyme activity but also specific increases in the proteins catalyzing the reactions. |
| Databáze: | OpenAIRE |
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