Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis
| Autor: | Kamran Nazmi, Yolanda Hornelas-Orozco, Adrian Canizalez-Roman, Hugo Aguilar-Diaz, Julio César Carrero, Francisco Gallardo-Vera, Claudia León-Sicairos, Jan G. M. Bolscher, Nidia León-Sicairos, Tomás Nepomuceno-Mejía |
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| Přispěvatelé: | Orale Biochemie (OII, ACTA), Oral Biochemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Giardiasis
0301 basic medicine Programmed cell death Cell Survival Peptide Biology Endocytosis Biochemistry Microbiology Flow cytometry Feces 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents SDG 3 - Good Health and Well-being medicine Animals Humans Trophozoites Propidium iodide Molecular Biology chemistry.chemical_classification 030102 biochemistry & molecular biology medicine.diagnostic_test Lactoferrin Cell Biology Peptide Fragments In vitro 030104 developmental biology Vacuolization chemistry biology.protein Cattle Giardia lamblia |
| Zdroj: | Biochemistry and Cell Biology, 95(1), 82-90. National Research Council of Canada Aguilar-Diaz, H, Canizalez-Roman, A, Nepomuceno-Mejia, T, Gallardo-Vera, F, Hornelas-Orozco, Y, Nazmi, K, Bolscher, J G M, Carrero, J C, Leon-Sicairos, C & Leon-Sicairos, N 2017, ' Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis ', Biochemistry and Cell Biology, vol. 95, no. 1, pp. 82-90 . https://doi.org/10.1139/bcb-2016-0079 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/bcb-2016-0079 |
| Popis: | Giardia intestinalis is the most common infectious protozoan parasite in children. Despite the effectiveness of some drugs, the disease remains a major worldwide problem. Consequently, the search for new treatments is important for disease eradication. Biological molecules with antimicrobial properties represent a promising alternative to combat pathogens. Bovine lactoferrin (bLF) is a key component of the innate host defense system, and its peptides have exhibited strong antimicrobial activity. Based on these properties, we evaluated the parasiticidal activity of these peptides on G. intestinalis. Trophozoites were incubated with different peptide concentrations for different periods of time, and the growth or viability was determined by carboxyfluorescein-succinimidyl-diacetate-ester (CFDA) and propidium iodide (PI) staining. Endocytosis of peptides was investigated by confocal microscopy, damage was analyzed by transmission and scanning electron microscopy, and the type of programmed cell death was analyzed by flow cytometry. Our results showed that the LF peptides had giardicidal activity. The LF peptides interacted with G. intestinalis and exposure to LF peptides correlated with an increase in the granularity and vacuolization of the cytoplasm. Additionally, the formation of pores, extensive membrane disruption, and programmed cell death was observed in trophozoites treated with LF peptides. Our results demonstrate that LF peptides exhibit potent in vitro antigiardial activity. |
| Databáze: | OpenAIRE |
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