Cloning and Characterization of a Mouse Type I Hair Keratin cDNA
| Autor: | Ian Sklaver, Arthur P. Bertolino, Theodore A. Schiff, Irwin M. Freedberg, Daniel M. Checkla, Robyn Notterman, Gary J. DiDona |
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| Rok vydání: | 1988 |
| Předmět: |
Type I keratin
Molecular Sequence Data Dermatology Biology Mouse Protein Biochemistry Hair keratin 03 medical and health sciences Mice 0302 clinical medicine Complementary DNA medicine Animals Amino Acid Sequence Cloning Molecular Peptide sequence Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences integumentary system Base Sequence cDNA library DNA Cell Biology Hair follicle Blotting Northern Molecular biology Amino acid medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Keratins Hair |
| Zdroj: | Journal of Investigative Dermatology. 91(6):541-546 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12476901 |
| Popis: | A cDNA library was prepared from poly(A)-containing C57BL/6J mouse hair-root-enriched mRNA. The library was screened using a radiolabeled nucleic acid probe prepared from a sheep wool, Type I keratin cDNA clone (SWK2). Clone MHKA-1 was shown to contain a mouse hair, Type I keratin cDNA insert by positive hybridization-selection translation assay, and by the corresponding deduced amino acid sequence. The size of the cDNA insert is 1585 bp (excluding homopolymer tails) and on the basis of Northern blot analysis it corresponds to a mRNA of approximately 1.6 Kb. The cloned cDNA sequence includes the entire coding region for a protein of 416 amino acids (including the initiation methionine). Comparison of the deduced amino acid sequence with that of sheep wool, Type I keratin (8c1) reveals an overall corresponding sequence identity of 87%. In contrast, the MHKA-1 protein is significantly less similar to non-hair Type I keratins. An additional 3 amino acids (adjacent proline residues) not present in the wool protein have been identified near the middle of the carboxy-terminal end of the mouse protein. MHKA-1 is the first of a series of mouse hair follicle cDNA clones to be identified and characterized that will enable us to study follicular regulatory mechanisms and the interrelationships among the proteins in the mammalian hair follicle. |
| Databáze: | OpenAIRE |
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