Prevalence and Antibiotic Resistance Patterns of Campylobacter spp. Isolated from Broiler Chickens in the North of Tunisia

Autor: Ahlem Jouini, Manel Gharbi, Awatef Béjaoui, Abderrazek Maaroufi, Chedia Aouadhi, Safa Hamrouni, Kais Ghedira, Chahrazed Zrelli, Abdeljelil Ghram, Cherif Ben Hamda, Ghaith Bessoussa
Přispěvatelé: Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire (LR11IPT03), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire de Bioinformatique, biomathématiques, biostatistiques (BIMS) (LR11IPT09), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Tunis El Manar (UTM), Faculté des Sciences de Bizerte [Université de Carthage], Université de Carthage - University of Carthage, This work was supported by the Tunisian Ministry of Higher Education and Scientific Research, Laboratory of Epidemiology and Veterinary Microbiology (LR16IPT03)., We would like to thank the farmers for having accepted to provide samples and to participate in this study as well as associated veterinarians for facilitating sample collection.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Veterinary medicine
Article Subject
MESH: Campylobacter/isolation & purification
medicine.drug_class
Tetracycline
030106 microbiology
Antibiotics
MESH: Poultry Diseases/drug therapy
lcsh:Medicine
MESH: Poultry Diseases/microbiology
Drug resistance
Biology
MESH: Genes
Bacterial/genetics
MESH: Microbial Sensitivity Tests/methods
medicine.disease_cause
MESH: Campylobacter/drug effects
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Antibiotic resistance
MESH: Campylobacter Infections/drug therapy
MESH: Anti-Bacterial Agents/pharmacology
medicine
MESH: Animals
MESH: Chickens/microbiology
MESH: Drug Resistance
Multiple
Bacterial/drug effects
MESH: Drug Resistance
Multiple
Bacterial/genetics
MESH: Prevalence
2. Zero hunger
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
General Immunology and Microbiology
Campylobacter
lcsh:R
General Medicine
MESH: Food Microbiology/methods
MESH: Campylobacter Infections/microbiology
Antimicrobial
3. Good health
MESH: Campylobacter/genetics
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Gentamicin
Flock
MESH: Tunisia
medicine.drug
Zdroj: BioMed Research International
BioMed Research International, Hindawi Publishing Corporation, 2018, 2018, pp.7943786. ⟨10.1155/2018/7943786⟩
BioMed Research International, Vol 2018 (2018)
ISSN: 2314-6133
2314-6141
DOI: 10.1155/2018/7943786⟩
Popis: The aim of the current study is to assess the prevalence ofCampylobacterinfection in broiler chickens, raised in intensive production conditions, and to evaluate the antimicrobial susceptibility of recoveredCampylobacterisolates. A total of 590 cloacal swab samples were taken from 13 broiler chicken flocks in the North East of Tunisia. All samples were tested for the presence of thermophilicCampylobacterby culture and PCR, targeting themapAandceuEgenes, respectively. Susceptibility to antimicrobial drugs was tested against 8 antibiotics. Prevalence ofCampylobacterinfection, relationship with geographic origins and seasons, antimicrobial resistance rates and patterns were analyzed. Total prevalence ofCampylobacterinfection in broiler flocks was in the range of 22.4%, with a predominance ofC. jejuni(68.9%), followed byC. coli(31.1%). Positive association was highlighted between the infection level and the season (P< 0.001), but no link was emphasized considering the geographic origin. Antimicrobial susceptibility testing revealed very high resistance rates detected against macrolide, tetracycline, quinolones, and chloramphenicol, ranging from 88.6% to 100%. Lower resistance prevalence was noticed forβ-lactams (47% and 61.4%) and gentamicin (12.9%). 17 R-type patterns were observed, and a common pattern was found in 30.3% of isolates. This study provides updates and novel data on the prevalence and the AMR of broiler campylobacters in Tunisia, revealing the occurrence of high resistance to several antibiotics and emphasizing the requirement of better surveillance and careful regulation of antimicrobials use.
Databáze: OpenAIRE
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