Evaluating the potential of effluents and wood feedstocks from pulp and paper mills in Brazil, Canada, and New Zealand to affect fish reproduction: chemical profiling and in vitro assessments
| Autor: | A.H. Slade, Jagmohan Kohli, Douglas A. Holdway, D. Scott Smith, Rodrigo Orrego, Mark R. Servos, Brendan D. Smith, Philip D. Scott, Brian I. O'Connor, Tibor Kovacs, Heidi Engelhardt, Vance L. Trudeau, Tatiana Heid Furley, John T. Arnason, Andrew Waye, Milestone Craig Brennan, L. Mark Hewitt, Deborah L. MacLatchy |
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| Rok vydání: | 2011 |
| Předmět: |
Paper
Canada Chromatography Gas Elemental chlorine free Fish reproduction Saccharomyces cerevisiae engineering.material Endocrine Disruptors complex mixtures Tandem Mass Spectrometry Goldfish Botany Environmental Chemistry Animals Effluent Monoamine Oxidase Waste Products Analysis of Variance business.industry Chemistry Glutamate Decarboxylase Pulp (paper) Reproduction digestive oral and skin physiology In vitro toxicology Paper mill Estrogens General Chemistry Pulp and paper industry Yeast Receptors Androgen engineering business Kraft paper Brazil Water Pollutants Chemical New Zealand |
| Zdroj: | Environmental sciencetechnology. 46(3) |
| ISSN: | 1520-5851 |
| Popis: | This study investigates factors affecting reproduction in fish exposed to pulp and paper mill effluents by comparing effluents from countries with varying levels of documented effects. To explore the hypothesis of wood as a common source of endocrine disrupting compounds, feedstocks from each country were analyzed. Analyses included in vitro assays for androgenic activity (binding to goldfish testis androgen receptors), estrogenic activity (yeast estrogen screen), and neurotransmitter enzyme inhibition (monoamine oxidase and glutamic acid decarboxylase). Chemical analyses included conventional extractives, known androgens, and gas chromatograph index (GCI) profiles. All effluents and wood contained androgenic activity, particularly in nonpolar fractions, although known androgens were undetected. Effluents with low suspended solids, having undergone conventional biotreatment had lower androgenic activities. Estrogenic activity was only associated with Brazilian effluents and undetected in wood. All effluents and wood inhibited neurotransmitter enzymes, predominantly in polar fractions. Kraft elemental chlorine free mills were associated with the greatest neurotransmitter inhibition. Effluent and wood GCI profiles were correlated with androgenic activity and neurotransmitter enzyme inhibition. Differences in feedstock bioactivities were not reflected in effluents, implying mill factors mitigate bioactive wood components. No differences in bioactivities could be discerned on the basis of country of origin, thus we predict effluents in regions lacking monitoring would affect fish reproduction and therefore recommend implementing such programs. |
| Databáze: | OpenAIRE |
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